豌豆种质资源耐盐性的鉴定与评价

Identification and Evaluation of Salt Tolerance of Pea Resources

【目的】为适应河北滨海地区豌豆生产需要。【方法】以32份豌豆种质资源为材料,混合盐胁迫豌豆萌发期,对豌豆种质的耐盐性进行鉴定和综合评价。【结果】混合盐溶液设置5个浓度梯度,当浓度升至150 mmol/L时,豌豆品种(系)间的相对发芽率标准差达到最大,为19.75。主成分分析中,相对发芽率、相对芽鲜重和相对发芽势的累计贡献率达到了80.18%。聚类分析根据耐盐性将32个豌豆种质资源分为4个级别,其中高耐盐豌豆品种(系)8个,耐盐豌豆品种(系)3个,中耐豌豆品种(系)7个,盐敏感豌豆品种(系)10个。【结论】豌豆具有耐盐性,不同豌豆品种耐盐性不同,150 mmol/L混合盐胁迫豌豆萌发期是豌豆耐盐性鉴定的有效方法。相对发芽率、相对芽鲜重和相对发芽势可构成豌豆耐盐性的评价指标体系。8个高耐盐豌豆品种(系)可作为亲本进一步培育抗逆豌豆优良品种或滨海盐碱地进行引种试验与示范推广。

【Objective】The current study was conducted to meet the needs of pea production in Hebei coastal area.【Method】32 pea germplasm resources were used as materials to identify and comprehensively evaluate the salt tolerance of pea germplasm under mixed sodium salt stress at germination stage.【Result】When the concentration of mixed salt solution increased to 150 mmol/L,the standard deviation of relative germination rate(RGR)among pea varieties(lines)reached the maximum,19.75.principal component analysis showed that the cumulative contribution rate of relative germination rate(RGR),relative shoot fresh weight(RSFW)and relative germination potential(RGP)reached 80.18%.The 32 pea germplasm resources were divided into four grades according to salt tolerance by cluster analysis,including 8 high salt tolerance varieties(lines),3 salt tolerance varieties(lines),7 medium tolerance varieties(lines)and 10 salt sensitive varieties(lines).【Conclusion】Pea has salt tolerance,and different pea varieties have different salt tolerance.150 mmol/L mixed salt stress at germination stage was an effective method to identify the salt tolerance of pea.Relative germination rate(RGR),relative shoot fresh weight(RSFW)and relative germination potential(RGP)can constitute the evaluation index system of salt tolerance of pea.Eight highly salt-tolerant pea varieties(lines)could be used as parents to further develop excellent varieties of stress-tolerant pea or to conduct introduction test and demonstration promotion in coastal saline-alkali land.