基于癌症基因图谱数据库分析Ephrin As在肝癌中的表达及评估肝癌发生的价值

Analysis of the expression of Ephrin As and its diagnostic value in hepatocellular carcinoma based on the cancer genome atlas dataset

目的通过对癌症基因图谱(TCGA)数据库中肝癌转录组数据集进行分析,寻找新的预测肝癌发生相关的基因。方法下载并纳入TCGA数据库中347例肝癌和50例正常癌旁对照的转录组与临床资料数据,进行Ephrin A1~5(EFNA1~5)mRNA表达差异分析。经logistic回归分析建立联合预测指标,并按肝癌StageⅠ~Ⅳ分组,分别与正常组进行比较,采用受试者工作特征(ROC)曲线评估Ephrin As(EFNAs)单项及多项联合在预测肝癌发生以及进展各个分期的价值。结果肝癌组EFNA1、EFNA3、EFNA4 mRNA表达水平高于正常组,EFNA2、EFNA5 mRNA表达水平低于正常组,差异有统计学意义(P<0.01)。EFNA3、EFNA4 mRNA联合预测肝癌发生的ROC曲线下面积(AUC)为0.923,敏感度为0.879,特异度为0.880;EFNA3、EFNA4 mRNA联合预测肝癌进展StageⅠ、Ⅱ、Ⅲ的ROC曲线下面积分别为0.908、0.946、0.938,均比单项EFNAs mRNA预测效能高。结论EFNAs可能作为预测肝癌发生的良好指标,且EFNA3、EFNA4联合预测肝癌发生有更好的价值。

Objective To investigate the new genes associated with the occurrence of hepatocellular carcinoma(HCC)by analyzing the transcriptome database downloaded from the cancer genome atlas(TCGA)database.Methods The mRNA expression profiles and associated clinical data,including 347 liver cancer tissues and 50 normal tissues,were obtained from TCGA database,and R software was used to explore the mRNA expression differences of Ephrin A1-5(EFNA1-5).The biomarker combined of multi EFNAs was established by logistic model.HCC group was divided into four group according to the StageⅠ-Ⅳ,and each group was compared to the normal group.Receiver operating characteristic(ROC)curve was used to access the diagnostic value of one Ephrin As(EFNAs)and such biomarker combined of multi EFNAs in predicating the occurrence and progression of HCC.Results The mRNA expressions of EFNA1,EFNA3 and EFNA4 in HCC group were higher than those in the normal group,while the mRNA expressions of EFNA2 and EFNA5 were lower than those in the normal group,the differences were statistically significant(P<0.01).The area under the curve(AUC)of the biomarker combined of EFNA3 and EFNA4 was 0.923,its sensitivity was 0.879 and specificity was 0.880.Furthermore,the AUC of the biomarker combined of EFNA3 and EFNA4 was 0.908,0.946,0.938 respectively in predication of HCC progression such as StageⅠ,Ⅱ,Ⅲ,which was higher than that of single EFNAs(P<0.01).Conclusion EFNAs maybe a valuable biomarker in predicating the occurrence of HCC,especially the synergistical predication biomarker combined of EFNA3 and EFNA4.