大豆开花期性状QTL定位和候选基因挖掘

QTL mapping and candidate genes identification for flowering time of soybean

大豆对光周期极为敏感,单一品种的适应范围狭窄。大豆品种在不同纬度间的适应性与开花期密切相关。为了获得更多的大豆开花期相关QTL,了解在豆适应机制,利用开花主效位点E1~E4基因型均相同的滑皮豆和齐黄26(E1e2asE3-HaE4)杂交衍生的重组自交系群体及前期基于特异长度扩增片段测序(Specific Length Amplified Fragment-sequencing,SLAF-seq)构建的高密度遗传图谱,对大豆开花期性状进行了QTL定位。共获得了分布在7条染色体上的11个QTL位点,其中4个位点(qFT8,qFT20-2,qFT14和qFT16)为本研究新发现的QTL。同时,研究发现6个QTL(qFT6-1,qFT8,qFT11-1,qFT19,qFT20-1和qFT20-2)在2013年和2014年两个环境中稳定存在。对稳定QTL位点间的基因进行生物信息学分析,筛选出4个可能参与开花期调控的候选基因。本研究结果能够为阐明大豆适应性分子机制和广适性分子育种提供一定的理论基础。

Soybean is sensitive to photoperiod,and the cultivation area of each soybean variety is restricted to a very narrow latitudinal range.The adaptability of soybean between different latitudes is closely related to flowering time.In order to excavate the genes controlling flowering time of soybean,we performed QTL mapping for flowering time by using recombinant inbred line(RIL)population derived from Huapidou×Qihuang 26 cross and high-density linkage map constructed based on specific length amplified fragment sequencing(SLAF-seq).In total,11 QTLs were identified on 7 chromosomes.Of them,4 QTLs(qFT8,qFT20-2,qFT14 and qFT16)were newly identified in this study.Furthermore,6 QTLs(qFT6-1,qFT8,qFT11-1,qFT19,qFT20-1 and qFT20-2)exist stably in 2 environments.By performing the sequences and bioinformatics analysis of genes between 6 stable QTL intervals,4 candidate genes related to flowering time regulation had been identified.The results of this research were expected to provide a foundation for understanding the molecular mechanism of soybean adaptability and cultivating the varieties with wide adaptability.