儿童白色糠疹的临床病理特征分析及其与氧化应激基因的关系研究

Clinicopathological Characteristics of Pityriasis Alyriasis in Children and Its Relationship with Oxidative Stress Genes

目的探讨儿童白色糠疹的临床病理特征及其与氧化应激基因的关系研究。方法随机纳入我院的白色糠疹患儿10例及10例健康体检儿童,使用RT-PCR方法检测患儿的氧化应激基因。结果氧化应激基因mRNA表达谱与正常组儿童的mRNA表达谱相比存在高显著差异的分子包括:SOD-1-mRNA,TH-mRNA,HBA mRNA(P<0.05),其中SOD-1-mRNA表达上调,TH-mRNA和HBA mRNA基因表达下调。白色糠疹组发病年龄以2~5岁为主,病程1~40个月,同时累及面部、躯干占20.0%。表皮角化过度7例(70.0%),角化不全2例,淋巴细胞侵入表皮3例,基底细胞液化2例,免疫组化T细胞标记物CD4阳性率为90.0%。相关分析显示,SOD-1-mRNA,TH-mRNA、HBA mRNA表达量与患儿年龄和CD4阳性表达有关(P<0.05),但与性别、部位和病程无关(P>0.05)。结论高显著差异表达的几种mRNA可能是儿童白色糠疹的潜在生物标志物,儿童白色糠疹可能与机体氧化有关。

Objective To investigate the clinicopathological characteristics of pityriasis alyriasis in children and its relationship with oxidative stress genes.Methods 10 children with pityriasis alyriasis and 10 healthy children were randomly enrolled in our hospital.Rt-pcr was used to detect the oxidative stress gene in the children.Results The mRNA expression profile of oxidative stress gene was significantly different from that of the normal group.Sod-1-mrna,TH-mRNA,HBA mRNA(P<0.05),soD-1-mrna expression was up-regulated,TH-mRNA and HBA mRNA gene expression was down-regulated.In the pityriasis alba group,the onset age was 2-5 years,the course of disease was 1-40 months,and the face and trunk were also involved,accounting for 20.0%.Epidermal hyperkeratosis in 7 cases(70.0%),keratosis in 2 cases,lymphocyte invasion in 3 cases,basal cell liquefaction in 2 cases,immunohistochemical T cell marker CD4 positive rate was 90.0%.Correlation analysis showed that the expression levels of SOD-1-mrna,TH-mrna and HBA mRNA were correlated with age and CD4 positive expression(P<0.05),but not with gender,site and course of disease(P>0.05).Conclusion The high and significantly differentially expressed mrnas may be potential biomarkers of pityriasis alyriasis in children,which may be related to oxidation.