SOX12在宫颈癌组织中的表达及生物学功能

The expression of SOX12 in cervical cancer tissues and biological functions

目的:探讨SRY盒转录因子12(SRY-box transcription factor 12,SOX12)在宫颈癌中的临床及生物学意义。方法:采用免疫组织化学染色检测50对宫颈癌及癌旁组织中SOX12的表达,评价SOX12表达水平与患者临床特征及预后的相关性。通过SOX12 shRNA质粒降低宫颈癌HeLa细胞内SOX12的表达水平,检测宫颈癌细胞的迁移及侵袭能力。结果:SOX12主要定位于细胞核中。与癌旁组织相比,宫颈癌组织中SOX12的表达水平明显升高(P=0.004)。SOX12高表达与淋巴结转移(P=0.020)及较晚的FIGO分期(P=0.038)密切相关,并且SOX12高表达的宫颈癌患者术后总生存期(HR=2.169,P=0.004)及无病生存期(HR=2.438,P=0.006)均较短。体外研究显示,沉默SOX12表达可以抑制HeLa细胞迁移(P=0.039)和侵袭(P=0.026)。结论:SOX12高表达与宫颈癌恶性临床特征及不良预后密切相关,SOX12具有抗肿瘤转移作用,SOX12作为治疗靶点存在研究价值。

Objective:To investigate the clinical significance and biological functions of SRY-box transcription factor 12(SOX12)in cervical cancer.Methods:The expression of SOX12 in 50 paired cervical cancer and matched adjacent tissues was detected by immunohistochemistry(IHC).Pearson Chi-square analysis and Kaplan-Merier analysis were used to analyze the relationship between SOX12 expression and clinical features.SOX12 expression was down-regulated in HeLa cells by shRNA plasmids.Cell migration and invasion in cervical cancer were measured by Transwell assay.Results:SOX12 expression was mainly localized in the nucleus.The expression of SOX12 was increased in cervical cancer tissues than adjacent tissues(P=0.004).High expression of SOX12 was associated with lymphatic metastasis(P=0.020)and advanced FIGO stage(P=0.038).Patients with high SOX12 expression had shorter overall survival time(HR=2.169,P=0.004)and disease-free survival time(HR=2.438,P=0.006).Down-regulation of SOX12 inhibited migration(P=0.039)and invasion(P=0.026)in HeLa cells.Conclusion:Increased expression of SOX12 in cervical cancer tissues associates with worse clinical features and worse survival.SOX12 inhibits tumor metastasis in vitro.