两个大豆ERD15基因的克隆、生物信息学分析及功能初步鉴定

Cloning,bioinformatics analysis and function identification of two soybean ERD15 genes

大豆ERD15(early responsive to dehydration 15)作为一种转录因子,能够与NRP-B启动子结合,激活NRP-B介导的细胞死亡反应。该家族包含6个成员,本文主要对其中两个成员GmERD15a和GmERD15b进行研究。通过对GmERD15a、GmERD15b基因的克隆、生物信息学分析、组织表达分析以及在酵母体系中的抗逆性鉴定,初步探究GmERD15a和GmERD15b基因的功能。结果表明,GmERD15a基因全长378 bp,GmERD15b基因全长318 bp;GmERD15a与GmERD15c相似性最高,其次是JrERD15;GmERD15b与GmERD15f相似性最高,其次是VaERD15;在线网站分析表明GmERD15a与GmERD15b均为亲水性蛋白;GmERD15a基因在20 d的胚中表达量最高,GmERD15b基因在根中的表达量最高;GmERD15a和GmERD15b基因对干旱胁迫和盐胁迫敏感。此研究为深入研究两基因的功能和作用机理提供理论依据。

ERD15(Early Responsive to Dehydration 15),as a transcription factor,binds to NRP-B promoter and activates NRP-B-mediated cell death.This family consists of six members,of which two members,GmERD15a and GmERD15b,are mainly studied in this study.The functions of GmERD15a and GmERD15b genes were preliminarily explored through cloning,bioinformatics analysis,tissue expression analysis and stress resistance identification in yeast transient system.The results show that the full length of GmERD15a is 378 bp,and the full length of GmERD15b is 318 bp.GmERD15a has the highest homology with GmERD15c,followed by JrERD15.GmERD15b has the highest homology with GmERD15f,followed by VaERD15.Both GmERD15a and GmERD15b are hydrophilic proteins.GmERD15a and GmERD15b were the highest expression levels in 20 d embryos and roots.GmERD15a and GmERD15b were sensitive to drought stress and salt stress.The study was expected to provide a theoretical basis for further study of the function and mechanism of the two genes.