豆科MIKC型MADS-box基因家族生物信息学分析

Bioinformatics analysis of MIKC-type MADS-box gene family in legumes

MIKC型MADS-box是一类生物功能丰富的转录因子家族,参与调控植物的生长发育。为深入研究豆科MIKC型MADS-box基因家族生物学特性,利用生物信息学方法在大豆和蒺藜苜蓿中分别鉴定出92和45个MIKC型基因,并将其分为15个亚类。蛋白基序分析发现,大豆与蒺藜苜蓿不同亚类的共同基序不同,基因结构发生变化;共线性分析及KS分析表明,大豆90.5%的基因对和蒺藜苜蓿87.1%的基因对产生于双子叶植物共同经历的三倍化事件之前;大豆基因表达模式分析表明,大豆幼苗期总体表达量高于其他时期,其中SVP、SOC1、AGL12亚类表达量较高;蛋白互作网络分析表明,大豆SVP蛋白与CO、FT和TFL1蛋白相互作用,一起调控植物开花发育。本研究为进一步揭示MADS-box家族基因的生物学功能奠定基础。

MIKC MADS-box is a biological function rich family of transcription factors,and involved in regulating plant growth and development.In order to study the biological characteristics of MIKC-type MADS-box family genes in legumes,92 and 45 MADS-box genes were identified in soybean and Medicago truncatula by bioinformatics method,and they were divided into 15 subfamilies.Motif analysis showed that the motifs of soybean and Medicago were different,and the gene structure and function changed.Collinearity and Ks analysis showed that 90.5%of the MIKC gene pairs in soybean and 87.1%of the MIKC-type gene pairs in Medicago were generated before the whole genome triplication in dicotyledons.Expression pattern analysis of genes in soybean showed that the MIKC MADS-box genes in soybean seedling stage were expressed higher than other stages,especially the expression of SVP,SOC1 and AGL12 subfamilies.Protein interaction network showed that soybean protein SVP interacted with CO,FT and TFL1 to regulate plant flowering.This study will lay a foundation for the further biological functions of MADS-box gene family.