核盘菌Ss160的基因克隆与功能初探

Cloning and functional analysis of Ss160 in Sclerotinia sclerotiorum

由核盘菌(Sclerotinia sclerotiorum)引起的菌核病是油菜等十字花科作物的重要病害,对油菜的产量品质造成严重影响。本研究基于转录组数据筛选到一个核盘菌基因Ss160(Sscle04g035160),发现Ss160在接种油菜的核盘菌中高度表达。生物信息分析表明该基因具有信号肽,且序列在真菌界高度保守。亚细胞定位显示Ss160无特异性的定位,烟草叶片瞬时表达Ss160在核盘菌离体叶接种实验中病斑扩展显著小于表达空载体的和野生型烟草叶片,暗示异源表达Ss160能够提高植物的菌核病抗性。基于酵母系统的体外实验证明Ss160具有转录激活能力。该研究结果为后续Ss160在核盘菌-植物互作中功能机制的研究提供基础,也为油菜菌核病的研究提供借鉴和参考。

The Sclerotinia disease,caused by the Sclerotinia sclerotiorum fungi,is an important disease in most of cruciferous plants including Brassica napus.The disease has a serious impact on the yield quality of B.napus.In this study,we screened a Sclerotinia gene,named Ss160(Sscle04g035160)based on the transcriptome analysis,and we found that Ss160 was highly expressed in S.sclerotiorum when inoculated them on leaves of B.napus.The bioinformatics analysis indicated that Ss160 contained a signal peptide and the amino sequence were well-conserved across fungi species.The subcellular location of Ss160 was not specific.Furthermore,the tobacco leaves transient expressed of Ss160,showed significant smaller disease lesion than the control,suggesting that heterologous expression of Ss160 in plant can enhance its Sclerotinia disease resistance.We further verified the transcript activation activity of Ss160 by yeast proteins interaction system in vitro.Our results lay the foundation for further research of the functional mechanisms of Ss160,and also provide the reference for Sclerotinia disease resistance study in B.napus.