外源褪黑素对菜用大豆残留百菌清和多菌灵降解的影响

Effect of exogenous melatonin on degradation of chlorothalonil and carbendazim residues in vegetable soybean

为研究外源褪黑素处理对菜用大豆残留杀菌剂降解的影响,以菜用大豆品种浙鲜12为材料,以多菌灵和百菌清为杀菌剂,通过测定外源褪黑素处理对菜用大豆农药残留量、抗氧化系统、解毒系统的影响,探讨外源褪黑素处理在菜用大豆农药降解中的作用。结果表明:外源褪黑素处理提高菜用大豆中抗坏血酸和谷胱甘肽含量以及抗氧化酶(超氧化物歧化酶、过氧化物酶、过氧化氢酶、抗坏血酸过氧化物酶)活性,有效清除菜用大豆体内活性氧,并且诱导了菜用大豆解毒相关基因的表达,增强其对残留多菌灵和百菌清的解毒代谢,从而促进残留农药的降解,降低菜用大豆中多菌灵和百菌清的残留量。

In order to study the effect of exogenous melatonin treatment on the degradation of pesticide residues in vegetable soybean,Zhexian12 was used as test material,chlorothalonil and carbendazim were used as test fungicides.Effects of exogenous melatonin treatment on pesticide residues,antioxidant system and detoxification system of vegetable soybean were determined to explore the effect of exogenous melatonin on the degradation of vegetable soybean pesticides.Results showed that exogenous melatonin increased the content of ascorbic acid and glutathione and activities of antioxidant enzymes(superoxide dismutase,peroxidase,catalase,ascorbate peroxidase)in vegetable soybean,effectively eliminated the reactive oxygen species in vegetable soybean,induced the expression of detoxification related genes of vegetable soybean,enhanced the detoxification metabolism of carbendazim and chlorothalonil,and promoted the degradation of residual pesticides,and thus reduced the residues of carbendazim and chlorothalonil in vegetable soybean.