毒毛花苷通过Nrf2抑制子宫内膜癌细胞增殖活性

Ouabain Inhibit the Proliferation of Endometrial Cells through Nrf2 Down-regulation

目的:通过研究毒毛花苷对核因子2相关因子2(nuclear factor erythroid 2-related factor 2,Nrf2)的抑制作用,深入探讨其抑制子宫内膜癌细胞增殖的作用机制。方法:(1)采用细胞增殖实验观察不同浓度的毒毛花苷对Ishikawa细胞增殖的影响;(2)通过克隆形成实验观察毒毛花苷对Ishikawa细胞增殖的作用;(3)采用蛋白免疫印迹法检测经毒毛花苷处理后,Ishikawa细胞Nrf2蛋白表达水平的变化。结果:经毒毛花苷处理后,Ishikawa细胞增殖受到显著抑制且呈剂量依赖性。Nrf2蛋白表达下调。结论:毒毛花苷可能通过下调Nrf2蛋白水平,从而抑制子宫内膜癌的恶性生物学行为。

Objective:By studying the inhibitory effect of Ouabain on nuclear factor erythroid 2-related factor 2(Nrf2),the mechanism of how it suppresses endometrial cancer cell proliferation is deeply explored.Methods:(1)Cell proliferation experiments were used to observe the effect of Ouabain on the proliferation of Ishikawa cells;(2)Colony formation assays were used to observe the effect of Ouabain on the proliferation of Ishikawa cells;(3)Western blotting assays were used to detect the changes of Nrf2 protein level in Ishikawa cells after treatment with Ouabain.Results:After treatment with Ouabain,the proliferation of Ishikawa cells was markedly inhibited in a concentration dependent manner,and the expression of Nrf2 protein was down-regulated.Conclusion:Our finding suggested that Ouabain may inhibit the malignant biological behavior of endometrial cancer by down-regulating Nrf2.