不孕症患者的子宫自然杀伤细胞、浆细胞与子宫内膜异位症的相关性

Association of Uterine Natural Killer Cells,Plasma Cells and Endometriosis in Infertile Patients

目的:探究不孕症患者的子宫自然杀伤细胞、浆细胞与子宫内膜异位症的相关性。方法:选择80例患有子宫内膜异位症的不孕症患者做为本研究的不孕症组和80例正常生育的健康女性做为本研究的健康组,对比分析两组患者人口学基本资料、子宫自然杀伤细胞(uNKs)、浆细胞(PC)水平、血清性激素水平、子宫内膜中血管内皮生长因子(VEGF)表达情况、免疫细胞水平。通过Person法分析不孕症患者的子宫自然杀伤细胞、浆细胞与子宫内膜异位症的相关性。结果:两组的BMI指数、流产史、吸烟史、饮酒史比较,差异有统计学意义(P<0.05);不孕症组患者uNKs、抗苗勒氏管激素(AMH)和黄体生成素(LH)水平均显著高于健康组;PC、卵泡刺激素(FSH)、雌激素(Estrogen)和性激素结合球蛋白(SHBG)、VEGF阳性表达水平、CD4^(+)、CD8^(+)和CD4^(+)/CD8^(+)水平均显著低于健康组,差异有统计学意义(P<0.05)。另外,不孕症患者的uNKs与子宫内膜异位症存在显著的正相关关系(r=1.555,P<0.001);PC与子宫内膜异位症存在显著的负相关关系(r=-3.778,P<0.001)。结论:不孕症患者的uNKs、PC参与不孕症的发生和发展过程,对女性子宫内膜异位症的诊断具有重要的参考意义。

Objective:To investigate the correlation between endometriosis and uterine natural killer cells,plasma cells in infertile patients.Methods:80 infertile patients with endometriosis were selected as the infertile group of this study and 80 healthy women with normal fertility were selected as the healthy group of this study.The basic demographic data,Natural killer cells(uNKs)and Plasma cells(Plasma cells)of the two groups were compared and analyzed.PC levels,serum sex hormone levels,expression of VEGF in endometri-um,immune cell level.The correlation between endometriosis and natural killer cells,plasma cells and endometriosis in infertile patients was analyzed by Person method.Results:BMI index,history of abortion,history of smoking and history of drinking were different between the two groups(P<0.05).uNKs,AMH and LH levels in infertile group were higher than those in healthy group.The positive expression levels of PC,FSH,SHBG,VEGF and CD4^(+),CD8^(+),CD4^(+)/CD8^(+)were lower than those in healthy group,the differences were statistically significant(P<0.05).In addition,there was a positive correlation between uterine uNKs and endometriosis in infertile patients(r=1.555,P<0.001).PC was negatively correlated with endometriosis(r=-3.778,P<0.001).Conclusion:uNKs and PC are involved in the occurrence and development of infertility,which has important reference significance for the diagnosis of female endometriosis.