蜂蜜中蜜源植物成分的微流体芯片检测方法研究

Study on microfluidic chip detection method of honey plant species in honey

目的 建立微流体芯片检测方法,用于蜂蜜掺伪的鉴别。方法 本研究使用纳米技术提取蜂蜜中的核酸,建立了包括8种蜜源植物和4种糖浆来源植物特征序列的微流体芯片检测方法,并对该方法的特异性进行测试。同时以桉树为例,对方法的灵敏度进行测试,并对收集的64个蜂蜜样品进行检测。结果 测试结果表明,该方法可以有效区分蜂蜜中12种植物成分,且桉树样本DNA含量(ng)的对数值与Ct值呈负相关关系,并初步得出其检出限约为1.0×10ng。64个样品中有47个样品提取出符合要求的DNA。使用微流体芯片结合荧光PCR技术可一次性从提出的核酸中检测蜂蜜中8种常见的蜜源植物成分和4种糖浆来源植物,15个样品检测结果为阳性。结论 该方法具有简便快捷、高灵敏度、高通量的特点,可以用于蜂蜜中蜜源植物检测和掺伪的鉴定。

Objective To establish a microfluidic chip detection method for the identification of honey adulteration.Methods The nucleic acid in honey was extracted by nanotechnology, and a microfluidic chip detection method including the characteristic sequences of 8 kinds of honey derived plants and 4 kinds of syrup derived plants was established, and the specificity of this method was tested. At the same time, the sensitivity of the method was tested with Eucalyptus as an example, 64 honey samples collected were tested. Results The test results showed that this method could effectively distinguish 12 kinds of plant components in honey, and the DNA content of Eucalyptus samples had a negative correlation with Ct value, and preliminarily concluded that the limit of detection was about 1.0×10ng. Forty-seven of the 64 samples were able to extract DNA that met the requirements. Microfluidic chip combined with fluorescence PCR technology could be used to detect 8 kinds of common honey plant species and 4 kinds of syrup plant components at one time, and 15 samples were all positive. Conclusion This method is quick, high sensitivity and high throughput, and can be used for the detection and identification of honey plant species in honey.