精神分裂症患者外周血免疫细胞线粒体质量变化的研究

Study on the mitochondrial mass of peripheral blood immune cells in patients with schizophrenia

目的分析精神分裂症患者与健康对照者外周血免疫细胞亚群及其线粒体质量变化特征的差异。方法选取2021年2—10月在泉州市第三医院门诊或住院治疗的20~70岁精神分裂症患者(精神分裂症组, 487例)及同期同年龄段健康体检人群(对照组, 175名)为研究对象。采用PANSS(Positive and Negative Syndrome Scale)评估患者的精神病理症状, 采用流式细胞技术检测外周血中T淋巴细胞及其亚群的百分比、绝对计数、线粒体质量(mitochondria mass)、单细胞线粒体质量(single cell mitochondria mass)的差异对比;为研究线粒体参数与临床表现是否一致, 对32例首次就诊患者进行治疗前后检测数据对比。使用人淋巴细胞线粒体功能分析系统(软件)进行线粒体检测结果校准, 采用SPSS软件进行统计学分析, 采用flowjo软件进行流式数据t-SNE降维分析。结果患者组 CD8+T细胞单细胞线粒体质量值均显著高于对照组(P<0.001), ROC曲线确定患者与健康人群临界值为CD8+T细胞单细胞线粒体质量>95.84。不同年龄段精神分裂症患者(20~70岁)CD8+T细胞单细胞线粒体质量均显著高于对照人群(P<0.05或P<0.001)。32例首次就诊精神分裂症患者治疗前后CD8+T细胞单细胞线粒体质量与PANSS数据呈线性正相关[P<0.05, ρ(Spearman)>0]。结论精神分裂症患者外周血中免疫细胞线粒体代谢异常, 单细胞线粒体质量显著上调。

Objective To analyze the differences in the variation characteristics of peripheral blood immune cell subsets and their mitochondrial mass between schizophrenia patients(SZ)and healthy individuals(HC).Methods The selected subjects included 487 schizophrenic patients aged 20 to 70(schizophrenia group),who were diagnosed and treated in Quanzhou Third Hospital from February 2021 to October 2021,and 175 healthy individuals(control group)of the same age and during the same period.Their age,gender,years of education,and years of illness were collected.The psychopathological symptoms of the patients were assessed by PANSS(Positive and Negative Syndrome Scale).The flow cytometry was used to analyze the percentage,the absolute count of T cells and their subsets in peripheral blood,the mitochondria mass(MM)and the variation in the single-cell mitochondria mass(SCMM)of these T cells.To determine whether the mitochondrial mass parameters were consistent with clinical manifestations,the test data collected from 32 first diagnosed patients were compared before and after treatment.The analysis methods involved in the study mainly include the Human Lymphocyte Mitochondrial Function Analysis System(HLMFAS)used to calibrate of the mitochondrial test results,the SPSS software used for statistical analysis,and the flowjo software used for the t-SNE dimension reduction analysis of the flow cytometry data.Results The SCMM value of CD8+T cells(abbreviated as CD8+)in SZ was significantly higher than those values in HC(P<0.001).The threshold of the ROC curve of the SCMM(CD8+)between SZ and HC was greater than 95.84.SZ showed a significantly higher SCMM(CD8+)value than HC across all age groups(20-70 years).For the 32 first diagnosed patients,the test data were monitored before and after treatment.The results indicated a positive linear correlation between the SCMM of CD8+and the PNASS data(P<0.05,ρ(Spearman)>0).Conclusion Schizophrenic patients have abnormal mitochondrial metabolism in their peripheral immune cells with an up-regulated SCMM,indicating possible damage to the mitochondria.