Dystrophin、MGF及M-Cad在难产大鼠模型盆底肌肉中的表达意义研究

Expression of Dystrophin,MGF and M-Cad in Pelvic Floor Muscles of Dystocia Rat Model and Its Significance

目的:研究模拟难产大鼠模型盆底肌肉中Dystrophin、MGF及M-Cad的表达,并探讨其表达与压力性尿失禁的相关性。方法:选择未生产过的雌性SD大鼠40只,按照随机数字表法均分为对照组、模型1组、模型2组和模型3组,对照组大鼠正常喂养,模型1组大鼠行阴道扩张手术,模型2组大鼠切除双侧坐骨神经,模型3组大鼠既行阴道扩张术又切除双侧坐骨神经。喂养1月后,称重后处死各组大鼠,并分离盆底肌肉,称重后通过定量PCR法(qPCR)检测Dystrophin、MGF及M-Cad m RNA的表达。结果:与对照组大鼠相比,难产大鼠模型组(模型1组、模型2组和模型3组)体重和盆底肌肉重量无显著变化(P>0.05),漏尿点压力和最大膀胱容量均显著下降(P<0.05),盆底肌肉Dystrophin、MGF及M-Cad m RNA表达均显著下降(P<0.05)。Pearson相关性分析:大鼠盆底肌肉Dystrophin、MGF及M-Cad m RNA表达水平与漏尿点压力均呈密切正相关(Dystrophin:r=0.725,P=0.002;MGF:r=0.702,P=0.018;M-Cad:r=0.838,P<0.001);鼠盆底肌肉Dystrophin、MGF及M-Cad m RNA表达水平与最大膀胱容量也均呈密切正相关(Dystrophin:r=0.733,P=0.001;MGF:r=0.682,P=0.025;M-Cad:r=0.819,P<0.001)。结论:模拟难产大鼠模型盆底肌肉Dystrophin、MGF及M-Cad的表达降低,并且提示其表达降低可能与压力性尿失禁有关。

Objective:To study the expression of Dystrophin,MGF and M-Cad in pelvic floor muscles of dystocia rat model,and to explore its relationship with stress urinary incontinence.Methods:Forty unborn female SD rats were selected and divided into 3 groups according to the random number table method:control group,model 1 group,model 2 group and model 3 group.The rats in the control group were fed normally,the rats in the model 1 group underwent guided dilatation surgery,the rats in the model 2 group had bilateral sciatic nerves removed,and the rats in the model 3 group underwent both vaginal dilation and bilateral sciatic nerve removal.After feeding for 1 month,the rats in each group were sacrificed after weighing,and the pelvic floor muscles were separated.After weighing,the expression of Dystrophin,MGF and M-Cad m RNA was detected by quantitative PCR(qPCR).Results:Compared with the control group,the body weight and pelvic floor muscle weight of the dystocia rat model group(model 1,model 2 and model 3) did not change significantly(P>0.05),and the leakage point pressure and maximum bladder volume were both significantly decreased(P<0.05),and the expression of Dystrophin,MGF and M-Cad m RNA in pelvic floor muscles were also both significantly decreased(P<0.05).Pearson correlation analysis:the expression levels of Dystrophin,MGF and M-Cad m RNA in the pelvic floor muscles of rats are closely related to the pressure of the leak point(Dystrophin:r=0.725,P=0.002;MGF:r=0.702,P=0.018;M-Cad:r=0.838,P<0.001);the expression levels of Dystrophin,MGF and M-Cad m RNA in rat pelvic floor muscles are also closely correlated with maximum bladder capacity(Dystrophin:r=0.733,P=0.001;MGF:R=0.682,P=0.025;M-Cad:r=0.819,P<0.001).Conclusion:The expression of Dystrophin,MGF and M-Cad in pelvic floor muscles of dystocia rat model decreased,and the decreased expression were thought to be related to stress urinary incontinence.