生驼乳中金黄色葡萄球菌的分离鉴定与耐药性分析

Isolation,Identification and Drug Resistance Analysis of Staphylococcus aureus from Raw Camel Milk

为了解生驼乳中金黄色葡萄球菌的污染状况,本文从骆驼养殖户中采集了90份生驼乳,对其进行金黄色葡萄球菌的增菌、培养和分离,并利用全自动微生物生化鉴定仪进行鉴定,同时对分离出的阳性菌株采用纸片扩散法进行耐药性分析。生化鉴定结果表明,90份生驼乳样本中分离出8株金黄色葡萄球菌,分离率8.9%。耐药性分析表明,8株阳性菌株对青霉素耐药率达75%;对氧氟沙星敏感率最高,可达100%;对头孢哌酮钠、环丙沙星敏感性较高,敏感率可达87.5%;对链霉素、卡那霉素、阿莫西林、头孢唑啉和头孢西丁,四环素、氯霉素、头孢噻肟、林可霉素、苯唑西林、庆大霉素较敏感,敏感率在37.5%~75.0%之间。本研究为对生驼乳中的金黄色葡萄球菌的污染防控提供了参考依据。

In order to understand the contamination status of Staphylococcus aureus in raw camel milk in the breeding process,this study collected 90 raw camel milk samples from camel farmers,and carried out the enrichment,culture and separation of Staphylococcus aureus,and the automatic microbial biochemical identification instrument was used for identification,and the drug resistance analysis of the isolated positive strains was carried out by the disc diffusion method.The results of biochemical identification showed that 8 strains of Staphylococcus aureus were isolated from 90 raw camel milk samples,with an isolation rate of 8.9%.The drug resistance analysis showed that the 8 positive strains were resistant to penicillin up to 75%,the sensitivity rate to ofloxacin was the highest,up to 100%,the sensitivity rate to cefoperazone sodium and ciprofloxacin was higher,and the sensitivity rate was up to 87.5%,the sensitivity rate to streptomycin,kanamycin,amoxicillin,cefazolin and cefoxitin,tetracycline,chloramphenicol,cefotaxime,lincomycin,benzacillin and gentamicin ranged from 37.5%to 75.0%.This study provides a reference for the prevention and control of Staphylococcus aureus in raw camel milk.