m~6A甲基化转移酶ZC3H13在胃癌组织和血清中的表达及临床意义

Expression and Clinical Significance of m6A Methylatransferase ZC3H13 in Gastric Cancer Tissues and Serum

目的 探讨m~6A甲基化转移酶ZC3H13在胃癌患者组织和外周血中的表达情况及其在胃癌中的应用价值。方法 基于生物信息学数据库UALCAN和GEPIA,从转录水平比较胃癌及癌旁正常组织中ZC3H13的表达差异;GEPIA和Kaplan-Meier Plotter法分析ZC3H13表达水平与胃癌患者总生存期的关系。ELISA法检测80例初诊胃癌患者和50例健康者ZC3H13的浓度,并分析其与临床病理特征的关系;IHC法检测74例癌组织以及40例非配对癌旁正常组织中ZC3H13的表达水平,分析其与临床病理特征的关系。结果 ZC3H13在胃癌组织中的表达明显高于癌旁正常组织(P<0.05)。胃癌组织中ZC3H13蛋白的阳性率显著高于癌旁正常组织(74.3% vs.52.5%,P<0.05)。胃癌患者外周血清的ZC3H13的浓度明显高于健康对照者(P<0.05)。胃癌组织和外周血清中的ZC3H13的表达水平均与患者的性别、分化程度、肿瘤分期、浸润深度有关(P<0.05)。多因素分析显示,ZC3H13表达不是胃癌患者预后不良的独立危险因素(P>0.05)。ROC曲线下面积为 0.826(P<0.05),诊断价值较好。血清ZC3H13蛋白浓度以4.87 ng/ml为临界值,此时ZC3H13对胃癌诊断的敏感度为98.8%,特异性为50.0%。结论 ZC3H13在胃癌组织中高表达,外周血清中ZC3H13浓度也显著升高。ZC3H13在胃癌发生发展中可能发挥重要作用,对胃癌诊断有一定的临床诊断价值。

Objective To investigate the expression of m6A methylatransferase ZC3H13 in tissues and peripheral blood of patients with gastric cancer and its application value in gastric cancer.Methods UALCAN and GEPIA databases were used to analyze the expression difference of ZC3H13 in gastric cancer and adjacent normal tissues at transcription level;GEPIA and Kaplan-Meier Plotter databases were used to analyze the correlation between ZC3H13 expression level and OS of gastric cancer patients.ELISA was used to determine the concentration of ZC3H13 in 80 newly-diagnosed gastric cancer patients and 50 healthy controls,and to analyze its relation with clinicopathological data;IHC method was used to detect the expression level of ZC3H13 in 74 cases of cancer tissues and 40 cases of unpaired paracancerous tissues,and to analyze its relation with clinicopathological data.Results The expression of ZC3H13 in gastric cancer tissues was significantly higher than that in adjacent gastric tissues(P<0.05).The positive rate of ZC3H13 protein in gastric cancer tissues was significantly higher than that in adjacent normal tissues(74.3%vs.52.5%,P<0.05).Serum ZC3H13 concentration in gastric cancer group was significantly higher than that in healthy control group(P<0.05).The expression level of ZC3H13 in gastric cancer tissues and peripheral blood was related to gender,differentiation degree,clinical stage and infiltration depth of gastric cancer(P<0.05).Multivariate analysis showed that ZC3H13 expression was not an independent risk factor for poor prognosis of gastric cancer patients(P>0.05).The AUC was 0.826(P<0.05),indicating good diagnostic value.The critical value of serum ZC3H13 protein concentration was 4.87 ng/ml,and the sensitivity and specificity of ZC3H13 in the diagnosis of gastric cancer were 98.8%and 50.0%.Conclusion ZC3H13 is highly-expressed in gastric cancer tissues,and the concentration of ZC3H13 protein in peripheral blood is also significantly increased.ZC3H13 may play an important role in the occurrence and development of gastric cancer,and has certain clinical diagnostic value for gastric cancer.