水解蛋白酶ZL-1在饲料鱼溶浆中的酶解应用

The enzymatic application of hydrolytic protease ZL-1 in feed fish slurry

鱼溶浆是鱼粉加工副产物,富含蛋白,浆液粘度大.酶解加工鱼溶浆有利于将大分子蛋白质水解成肽和氨基酸等,降低浆液粘度,从而提高鱼溶浆的营养性及加工运输性.采用单因素实验和正交实验分析法系统研究并优化了利用ZL-1酶解鱼溶浆的工艺条件.得到的最佳酶解条件为:浆料浓度为10%,温度为55℃,不调节p H,ZL-1的加酶量为0.75%干物质浓度,酶解时间为2 h.最佳的工艺实验条件的验证结果表明,水解液中的氨基氮含量比优化前提升了129.47%,粘度降低了84.83%,用酶处理鱼溶浆显著增加了其流动性.

Fish slurry is a by-product of fish meal processing,rich in protein and with a high viscosity.The enzymatic processing of fish slurry facilitates the hydrolysis of large molecules of protein into peptides and amino acids etc,which reduces the viscosity of the slurry,thereby improving its nutritional and processing transportability.The process conditions for the enzymatic digestion of fish slurry using ZL-1 were systematically investigated and optimised using single factor tests and orthogonal analysis.The optimum enzymatic conditions were obtained as follows,slurry concentration of 10%,temperature of 55 ℃,no pH adjustment,enzyme addition of ZL-1 at 0.75%dry matter concentration and enzymatic digestion time of 2 h.The validation of the optimum process conditions showed that the amino nitrogen content of the hydrolysate increased by 129.47% compared to the pre-optimisation period.The viscosity was reduced by 84.83% and the treatment of fish slurry with enzymes significantly increased their fluidity.