心理应激致血浆外泌体miR-184-3p抑制血管内皮细胞增殖、迁移功能的作用分析

Effects of chronic unpredictable mild stress induced plasma exosomal miR-184-3p on proliferation and migration of vascular endothelial cells

目的探讨心理应激源性血浆外泌体miR-184-3p在调控血管内皮细胞增殖、迁移功能中的作用。方法(1)将60只6~8周龄C57BL/6雄性小鼠随机分为对照组和心理应激组,每组30只。采用21 d慢性不可预知性心理应激刺激建模。通过行为学实验(旷场实验、高架十字迷宫实验和强迫游泳实验)评价建模效果。提取血浆外泌体进行电镜分析,qPCR检测血浆外泌体miR-184-3p表达水平。(2)体外培养小鼠肺微血管内皮细胞(MPVEC),分别转染对照组小鼠血浆外泌体(C-EXO组)、心理应激组小鼠血浆外泌体(S-EXO组)、NC mimic组和miR-184-3p mimic组。划痕实验分析细胞迁移情况,EdU染色分析细胞增殖情况。结果(1)心理应激组小鼠探索行为较对照组显著降低,不动时间显著增加(P<0.01)。电镜分析验证血浆外泌体直径为30~200 nm的双层膜囊泡结构,qPCR证实心理应激组小鼠血浆外泌体中miR-184-3p的表达量显著增加(P<0.05)。(2)细胞实验结果显示S-EXO组细胞迁移和增殖能力较C-EXO组下降(P<0.01);同时miR-184-3p mimic组细胞增殖和迁移能力较NC mimic组下降(P<0.05)。结论慢性心理应激源性血浆外泌体可以通过介导miR-184-3p抑制血管内皮细胞的增殖和迁移。

Objective To investigate the role of psychological stressor plasma exosome miR-184-3p in regulating the proliferation and migration of vascular endothelial cells.Methods(1)Sixty male C57BL/6 mice aged 6 to 8 weeks were randomly divided into the control group and the psychological stress group,with 30 mice in each group.The model of 21 d chronic unpredictable psychological stress was used.Behavioral experiments(open field experiment,elevated cross maze experiment and forced swimming experiment)were used to evaluate the modeling effect.Plasma exosomes were extracted and analyzed by electron microscopy.The expression level of plasma exosome miR-184-3p was detected by qPCR.(2)Mouse lung microvascular endothelial cells(MPVEC)were cultured in vitro and transfected with plasma exosome of control mice(C-EXO group),plasma exosome of psychological stress group(S-EXO group),NC mimic and miR-184-3p mimic,respectively.Cell migration was analyzed by scratch test,and cell proliferation was analyzed by EdU staining.Results(1)Compared with the control group,the exploration behavior of mice in the psychological stress group was significantly decreased,and the immobility time was significantly increased(P<0.01).The structure of bilayer membrane vesicles with plasma exosomes diameter of 30-200 nm was verified by electron microscope analysis.qPCR confirmed that the expression level of miR-184-3p in plasma exosomes of mice in the psychological stress group was significantly increased(P<0.05).(2)The results of cell experiment showed that the migration and proliferation of the S-EXO group were lower than that of the C-EXO group(P<0.01).Meanwhile,the proliferation and migration of the miR-184-3P mimic group were decreased compared with the NC mimic group(P<0.05).Conclusion Chronic psychological stressor plasma exosomes can inhibit the proliferation and migration of vascular endothelial cells by mediating miR-184-3p.