三味山姜属中药黄酮类成分对胃溃疡寒证大鼠药效及作用机制研究

Pharmacological Effect and Mechanism of Flavonoids in Three Alpinia Herbal Medicines on Gastric Ulcer Rats of Cold Syndrome

目的:观察高良姜、大高良姜、红豆蔻3味山姜属中药黄酮类成分的温中散寒药效及作用机制,探讨“亲缘关系-活性成分-效用”间的关联性。方法:给予大鼠灌服冰知母水煎液与15%冰乙酸制备胃溃疡寒证模型,观察3味山姜属中药黄酮类成分对大鼠行为体征的影响,检测血清中一氧化氮(NO)、内皮素(ET)、血小板活化因子(PAF)、血栓素B(TXB)、血清皮质素(COR)的含量。结果:与正常组比较,模型组毛发脏乱、精神状态较差,体质量、进食量、饮水量减少,肛温降低;NO含量减少,ET、PAF、TXB、COR含量增加(P<0.01)。与模型组比较,高良姜、大高良姜高剂量组体质量增加(P<0.05);大高良姜高剂量组、红豆蔻高低剂量组进食量增加(P<0.05或P<0.01);高良姜、大高良姜、红豆蔻高剂量组饮水量增加,肛温升高,毛发及精神状态恢复(P<0.05)。高良姜高低剂量组、大高良姜与红豆蔻高剂量组NO含量增加(P<0.05或P<0.01)、COR含量减少(P<0.05或P<0.01);各给药组高低剂量组ET含量均减少(均P<0.05或P<0.01);高良姜与红豆蔻高剂量组、大高良姜高低剂量组PAF、TXB含量减少(P<0.05或P<0.01)。各给药组饮水与进食量、NO、ET含量比较,差异均有统计学意义(均P<0.05或P<0.01)。结论:3味山姜属中药黄酮类成分有抗胃溃疡寒证作用,药效之间存在一定的相关性和差异性;其作用机制可能与血清中NO含量增加,ET、PAF、TXB、COR含量减少有关。

Objective:To observe the middle-warming and cold-dissipating pharmacological effect and mechanism of flavonoids in three Alpinia herbal medicines:Rhizoma Alpiniae Officinarum(RAO),Rhizoma Alpiniae Galanga(RAG)and Fructus Alpiniae Galangae(FAG),and to explore the relationship between“genetic relationship-active ingredient-effect”of Chinese herbal medicines.Methods:Rats were given cold water decoction of Rhizoma Anemarrhenae and 15%glacial acetic acid to prepare a cold-syndrome gastric ulcer model.The effect of flavonoids in the three Alpinia herbal medicines on the behavioral signs of rats was observed,and the serum content of nitric oxide(NO),endothelin(ET),platelet-activating factor(PAF),thromboxane B(TXB),and cortisol(COR)was determined.Results:Compared with the normal group,the model group had dirty and messy hair,poor mental state,decreased body weight,average daily food intake and water intake,and lowered rectal temperature,and the content of NO was decreased while the content of ET,PAF,TXB,and COR was increased(P<0.01).Compared with the conditions in the model group,the body weight of RAO high-dose group and RAG high-dose group was elevated(P<0.05);food intake was enhanced in RAG high-dose group and FAG high-and low-dose groups(P<0.05 or P<0.01);water intake and rectal temperature in RAO,RAG and FAG high-dose groups were increased,with recovered hair and mental status(P<0.05);the content of NO in RAO high-and low-dose groups as well as RAG and FAG high-dose groups was elevated(P<0.05 or P<0.01)while the content of COR was reduced(P<0.05 or P<0.01).The content of ET in all high-and low-dose administration groups was lowered(P<0.05 or P<0.01),and there were significant differences in water and food intake and content of NO and ET(P<0.05 or P<0.01)in the administration groups.The RAO and FAG high-dose groups and RAG high-and low-dose groups had reduced content of PAF and TXB.Conclusion:Flavonoids in three Alpinia herbal medicines had the effect of resisting cold-syndrome gastric ulcer,and there were certain correlations and differences between the drug effects.The mechanism might be related to the increase of NO content and the decrease of ET,PAF,TXB,and COR content in serum.