偏痛汤1号对硝酸甘油致偏头痛模型大鼠药效学影响研究

Pharmacodynamical Effect of Piantongtang No.1 on Rat with Migraine Induced by Nitroglycerin

目的:研究硝酸甘油致大鼠偏头痛样疼痛行为的模型评价及偏痛汤1号对本模型药效学影响。方法:采用硝酸甘油颈部皮下注射方法造模。成模大鼠随机分成模型组、阳性药组[0.25 mg/(kg·d)]、TRPV1抑制剂组[TRPV1抑制剂,3 mg/(kg·d)]、偏痛汤1号组[13.5 g/(kg·d)]、偏痛汤1号+TRPV1抑制剂组,另设假手术组、空白组,每组12只,雌雄各半。各组分别用药干预1周,同时检测行为学及痛阈值变化。末次给药1 h后取血,采用酶联免疫吸附试验法检测组胺、5-羟色胺表达水平。结果:与空白组比较,模型组挠头及往返运动等行为评分均显著上升,而爬笼行为仅于第2次造模后评分显著增加,痛阈值均显著下降(P<0.05,P<0.01)。与模型组比较,偏痛汤1号组灌胃后挠头评分及血浆组胺含量均明显下降,痛阈值及5-羟色胺含量均显著上调,往返运动仅于第1天见偏痛汤1号组显著降低(P<0.05,P<0.01)。与TRPV1抑制剂组比较,偏痛汤1号组第6天痛敏反应不甚显著(P<0.05)。结论:实验成功复制大鼠偏头痛模型,尤以雌性模型变化显著。而偏痛汤1号对模型大鼠表现的偏头痛样行为、痛敏反应及血浆血管活性胺变化改善明显。

Objective:To study the model evaluation of nitroglycerin(NTG)-induced migraine-like pain behavior in rats and explore the pharmacodynamical effect of Piantongtang No.1(Pttn1)on the rat model of migraine.Methods:The model was induced by subcutaneous injection of NTG in the neck of rats.The rats with successful models were randomly divided into a model group,a positive drug group[0.25 mg/(kg·d)],a transient receptor potential cation channel subfamily V member 1(TRPV1)inhibitor group[3 mg/(kg·d)],a Pttn1 group[13.5 g/(kg·d)],a Pttn1+TRPV1 R group,a sham operation group,and a control group,with 12 rats(half male and half female)in each group.Each group was treated with drugs for 1 week,and the behavior and pain threshold changes were detected at the same time.Blood was taken 1 h after the last administration,and the expression levels of histamine(HA)and 5-hydroxytryptamine(5-HT)were determined by enzyme-linked immunosorbent assay(ELSIA).Results:As compared with the control group,the scores of behaviors such as head scratching and to and fro movement in the model group increased,while the score of cage climbing behavior only increased significantly after the second modeling and the pain threshold decreased(P<0.05,P<0.01).As compared with the model group,the score of head scratching and plasma HA content in the Pttn1 group decreased after intragastric administration,the pain threshold and 5-HT content up-regulated,and the to and fro movement was reduced in the Pttn1 group only on the first day(P<0.05,P<0.01).As compared with the TRPV1 inhibitor group,the hyperalgesia of the Pttn1 group was not significant on the 6 th day(P<0.05).Conclusion:This experiment successfully replicates the rat model of migraine,and the female rat model has significant changes.The Pttn1 obviously improves the migraine-like behavior,hyperalgesia,and plasma vasoactive amine changes in the rat model.