摘要
Regenerating periodontal bone tissues in the aggravated inflammatory periodontal microenvironment under diabetic conditions is a great challenge.Here,a polydopamine-mediated graphene oxide(PGO)and hydroxyapatite nanoparticle(PHA)-incorporated conductive alginate/gelatin(AG)scaffold is developed to accelerate periodontal bone regeneration by modulating the diabetic inflammatory microenvironment.PHA confers the scaffold with osteoinductivity and PGO provides a conductive pathway for the scaffold.The conductive scaffold promotes bone regeneration by transferring endogenous electrical signals to cells and activating Ca2+channels.Moreover,the scaffold with polydopamine-mediated nanomaterials has a reactive oxygen species(ROS)-scavenging ability and anti-inflammatory activity.It also exhibits an immunomodulatory ability that suppresses M1 macrophage polarization and activates M2 macrophages to secrete osteogenesis-related cytokines by mediating glycolytic and RhoA/ROCK pathways in macrophages.The scaffold induces excellent bone regeneration in periodontal bone defects of diabetic rats because of the synergistic effects of good conductive,ROS-scavenging,anti-inflammatory,and immunomodulatory abilities.This study provides fundamental insights into the synergistical effects of conductivity,osteoinductivity,and immunomodulatory abilities on bone regeneration and offers a novel strategy to design immunomodulatory biomaterials for treatment of immune-related diseases and tissue regeneration.
基金
grants from Sichuan Key Research and Development Program of China(22ZDYF2034)
National Natural Science Foundation of China(grant no.82072071,82072073)
Key-Area Research and Development Program of Guang Dong Province(2019B010941002)
Shenzhen Funds of the Central Government to Guide Local Scientific and Technological Development(2021SZVUP123)
Sichuan Science and Technology Program(2020YFS0170)
Fundamental Research Funds for the Central Universities(2682020ZT79)
Guangdong Basic and Applied Basic Research Foundation(2021B1515120019)
Research and Develop Program,West China Hospital of Stomatology Sichuan University(RD-03-202012,RD-03-202101).