摘要
This research was directed to understand the bactericidal effect of epidermal mucus of two Asian cat fish species viz.Clarias batrachus and Heteropneustes fossilis.Epidermal mucus extracts(raw and diluted)of both cat fish species were tested against several Gram negative(Pseudomonas aeruginosa,Escherichia coli,Klebsiella pneumonia,A.hydrophila)and Gram positive bacterial strains(Bacillius cereus,Staphylococcus aureus,S.epidermidis)and antibacterial results were also compared with two standard antibiotics viz.amikacin and chloramphenicol used as positive control.An A.hydrophila challenge experiment was also performed on all selected test fish species to examine the change in the amount of mucus production and its bactericidal impact..Both epidermal mucus extracts(raw and diluted)of all selected normal and bacterial challenged test objects showed potent bactericidal effect against all pathogenic bacterial strains taken under study.However,former was more effective than later.Also raw epidermal mucus extracts of both normal and bacterial challenged cat fish species exhibited significantly higher ZOI values against all selected microbial strains than diluted mucus extracts and antibiotic chloramphenicol.Hence,these outcomes have clearly revealed that this cost effective natural product acquired from fishes is the key component of their defensive system.Therefore,it could be utilized as a novel‘antimicrobial’in human as well as veterinary sector for combating against several bacterial diseases.
