艾叶不同极性溶剂提取物的抗氧化能力的研究

Study on antioxidant ability of different polarity solvent extracts of Artemisia argyi

试验旨在研究艾叶非挥发性成分不同极性溶剂提取物的抗氧化活性,探究艾叶非挥发性提取物抗氧化活性与多酚、黄酮以及多糖含量的关系。采用60~90℃石油醚去除艾叶中的脂溶性色素与挥发性物质,残渣分别以乙酸乙酯、正丁醇、乙醇、水逐级提取。使用铁离子还原法(FRAP)和1,1-二苯基-2-三硝基苯肼(DPPH)法测定4种提取物的抗氧化活性,将抗氧化活性转化为奎诺二甲基丙烯酸(Trolox)当量,测定提取物中的多酚、黄酮、多糖含量。结果显示:在水相、乙醇相、正丁醇相以及乙酸乙酯相中,多酚、黄酮、多糖主要富集在水相和乙醇相中,黄酮含量能够直接影响艾叶的抗氧化活性。研究表明,艾叶非挥发性提取物的抗氧化活性主要存在于水相和乙醇相中,黄酮类物质是抗氧化活性的主要成分,可将其作为天然抗氧化活性成分进行深度开发。

The experimet was to study the antioxidant activity of non-volatile components in Artemisia argyi leaves with different polarity solvent extracts and their antioxidant capacity, to investigate the relationship between antioxidant activity of non-volatile extract of Artemisia argyi leaf and content of polyphenols, flavonoids and polysaccharides. The lipid soluble pigment and volatile substances in Artemisia argyi leaves were removed by petroleum ether at boiling range of 60~90 ℃, the residues were extracted by ethyl acetate, n-butanol, ethanol and water, respectively. The antioxidant activities of four extracts were determined by ferric ion reduction method(FRAP) and 1, 1-diphenyl-2-trinitrophenylhydrazine(DPPH) method. The antioxidant activities were converted into Trolox equivalent, determining the content of polyphenols,flavonoids and polysaccharides in extract. The results showed that in water phase, ethanol phase, n-butanol phase and ethyl acetate phase, polyphenols, flavonoids and polysaccharides were mainly enriched in water phase and ethanol phase.The content of flavonoids directly could affect the antioxidant activity of polyphenols. The study indicates that the antioxidant activity of non-volatile extracts of Artemisia argyi leaves mainly exists in aqueous and ethanol phases, and flavonoids are the main components of antioxidant activity, which can be further developed as natural antioxidant active components.