胰岛素样生长因子结合蛋白-6在乳腺癌组织中的表达及临床意义

Expression and clinical significance of insulin-like growth factor binding protein-6 in breast cancer

目的探讨胰岛素样生长因子结合蛋白-6(IGFBP-6)在乳腺癌不同区域组织中的表达及临床意义。方法选取乐山市中区人民医院和湖北省中西医结合医院2019年1月至2021年12月收治的30例乳腺癌组织样本作为研究对象,根据乳腺癌组织距癌巢中心的区域位置不同分为3组,癌巢中心组、癌旁组、癌旁边界组。采用蛋白质印迹法(Western blot)和免疫组织化学法检测IGFBP-6及细胞核增殖抗原(Ki-67)表达,分析乳腺癌不同病理类型的各区域IGFBP-6、Ki-67在乳腺癌组织中的表达及病理特征的关系,两组比较采用Student’st检验。结果30例女性乳腺癌病例,病理Ⅰ级2例,Ⅱ级11例,Ⅲ级17例。Luminal A型7例、Luminal B型6例、人表皮生长因子受体2(Her-2)过表达型8例、Basical-like型9例。癌巢中心组IGFBP-6阳性率低于癌旁组及癌旁边界组,差异有统计学意义[癌巢中心组IGFBP-6的阳性率4例(13.3%),癌旁组23例(76.6%)和癌旁边界组26例(86.6%),χ2=18.23,P<0.05]。癌巢中心组Ki-67表达阳性率高于癌旁组及癌旁边界组,差异有统计学意义(χ2=23.78,P<0.05)。病理Ⅰ级阳性率显著高于Ⅱ级和Ⅲ级,差异有统计学意义(χ2=18.23,P<0.05)。Luminal B型阳性率高于Basical-like型,Luminal A型及Her-2过表达型,差异有统计学意义(χ2=4.61,P<0.05)。病理Ⅲ级Ki-67阳性率显著高于Ⅱ级和Ⅰ级,差异有统计学意义(χ2=14.26,P<0.05),而分子病理分型结果中Basical-like型Ki-67阳性率高于Luminal B型,Her-2过表达型及Luminal A型,差异有统计学意义(χ2=4.78,P<0.05)。结论IGFBP-6在乳腺癌癌巢组织中呈低表达,其表达水平与Ki-67和病理分级呈负相关。

Objective To investigate the expression of insulin-like growth factor binding protein-6(IGFBP-6)in different regions of breast cancer and its relationship with clinica significance.Methods Totally,30 breast cancer tissue samples from Leshan Central District People’s Hospital and Hubei Integrated Traditional Chinese and Western Medicine Hospital from January 2019 to December 2021 were selected as the research objects.According to the regional location of breast cancer tissue from the cancer nest center,they were divided into three groups:cancer nest center group,cancer adjacent group and cancer adjacent boundary group.Western blotting and immunohistochemistry were used to detect the expression of IGFBP-6 and proliferation cell nuclear antigen(Ki-67),and to analyze the relationship between the expression of IGFBP-6 and Ki-67 and pathological characteristics in different pathological types of breast cancer.Student’s t test was used for comparison between the two groups.Results There were 30 cases of female breast cancer,including 2 cases of pathological gradeⅠ,11 cases of pathological gradeⅡand 17 cases of pathological gradeⅢ.There were 7 cases of luminal type A,6 cases of luminal type B,8 cases of human epidermal growth factor receptor-2(Her-2)overexpression and 9 cases of basic like type.The positive rate of IGFBP-6 was 4 cases(13.3%)in the cancer nest center group,23 cases(76.6%)in the paracancerous group and 26 cases(86.6%)in the paracancerous boundary group(χ2=18.23,P<0.05).The positive rate of Ki-67 expression in the cancer nest center group was significantly higher than that in the adjacent group and adjacent boundary group(χ2=23.78,P<0.05).The positive rate of pathological gradeⅠwas significantly higher than that of pathological gradeⅡandⅢ,and the difference was statistically significant(χ2=18.23,P<0.05).The positive rate of luminal type B was significantly higher than that of basic like type,luminal type A and Her-2 overexpression type(χ2=4.61,P<0.05).The positive rate of Ki-67 in pathological gradeⅢwas significantly higher than that in gradeⅡand gradeⅠ,and the difference was statistically significant(χ2=14.26,P<0.05),while the positive rate of basic like Ki-67 was higher than that of luminal B,Her-2 overexpression and luminal A(χ2=4.78,P<0.05).Conclusion IGFBP-6 is low expressed in breast cancer nests,and its expression level is negatively correlated with Ki-67 and pathological grade.