摘要
建立基于一测多评法(quantitative analysis of multi-components by single marker, QAMS)的补骨脂含量测定方法,并进一步完善、提高其薄层鉴别方法。采用超高效液相色谱法,以补骨脂素为内参物,建立一测多评法同时测定补骨脂苷、异补骨脂苷、补骨脂素、异补骨脂素的含量,通过与外标法含量测定结果的比较分析,证明了构建的QAMS耐用性好、准确性高。依据补骨脂现行质量标准,进一步优化和完善补骨脂薄层鉴别方法,在原有补骨脂素、异补骨脂素为质控指标的基础上增加补骨脂酚的鉴别。该研究有助于补骨脂质量控制方法的完善和提高。
The present study established a determination method of Psoraleae Fructus by quantitative analysis of multi-components by the single marker(QAMS) and further improved the thin-layer chromatography(TLC) method. The QAMS method was established by UPLC with psoralen as the internal marker, and the content of psoralenoside, isopsoralenoside, psoralen, and isopsoralen was simultaneously determined. As revealed by the comparison with results of the external standard method, the QAMS method was accurate and feasible. According to the current quality standards of Psoraleae Fructus, the TLC method was further optimized and improved, and bakuchiol was added for identification based on the original TLC method with psoralen and isopsoralen as indicators. This study provides a reference for improving the quality control method of Psoraleae Fructus.
作者
王丹妮
郭佳铭
柴欣
杨静
王跃飞
WANG Dan-ni;GUO Jia-ming;CHAI Xin;YANG Jing;WANG Yue-fei(Tianjin Key Laboralory of TCM Chemistry and Analysis,State Key Laboratory of Component-bused Chinese Medicine,Instirute of Tradional Chinese Medicine,Tanjin Unitersily of Trouditionul Chinese Medicine,Tianjin 301617,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第15期4089-4097,共9页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2018YFC1707904)。
关键词
补骨脂
质量控制
一测多评法
薄层鉴别
Psoraleae Fructus
quality control
quantitative analysis of multi-components by single marker(QAMS)
TLC identification