小麦UDP-Ara变位酶基因TaUAM3的鉴定及表达特征分析

Identification and Expression Characteristics Analysis of a UDP-arabinopyranose Mutases Gene Ta UAM3 in Wheat

为解析小麦UDP-Ara变位酶在胁迫应答和信号转导中的功能,本研究获得了一个小麦UDP-Ara变位酶基因,命名为TaUAM3。序列分析结果表明,TaUAM3基因开放阅读框全长为1071 bp,编码356个氨基酸。通过生物信息学分析表明,TaUAM3基因定位于小麦3BL染色体上,其编码蛋白存在于细胞质中。通过对氨基酸序列多重比对分析表明,TaUAM3基因编码的氨基酸与其他单子叶植物UDP-Ara变位酶基因编码的氨基酸具有较高的同源性,其中与大麦HvUAM3的同源性最高,达95.1%。实时定量PCR分析表明,TaUAM3在小麦根、茎、叶和穗部均有表达,其中在茎部表达量最低,在根部表达量最高。植物激素水杨酸(SA)、茉莉酸甲酯(MeJA)、脱落酸(ABA)和乙烯(ETH)及NaCl盐胁迫均能诱导该基因的表达量迅速增加,表明TaUAM3可能参与了激素信号转导途径以及小麦对盐胁迫的响应。本研究为深入探讨UDP-Ara变位酶基因在植物响应逆境胁迫中的作用及分子机制提供了理论依据。

In order to analyze the function of wheat UDP-arabinopyranose mutases genes in stress response and signal transduction,a full length c DNA of a UDP-arabinopyranose mutases gene(named TaUAM3)was isolated from wheat.The sequence analysis results showed that the full length of the open reading frame of TaUAM3 was1071 bp encoding 356 amino acids.A bioinformatic analysis indicated that the TaUAM3 gene was located on the3BL chromosome of wheat and its coding protein was present in the cytoplasm.Multiple alignment analysis based on the amino acids indicated that TaUAM3 had high homology to UDP-arabinopyranose mutases genes from other monocot plants,and the highest similarity of 95.1%to HvUAM3 of barley.Quantitative real-time PCR(RT-qPCR)assay revealed that the Ta UAM3 gene was expressed in the roots,leaves,stems and spikes of wheat,but the lowest expression was in the stems and the highest expression was in the roots.TaUAM3 gene can rapidly respond to salicylic acid(SA),methyl jasmonate(MeJA),abscisic acid(ABA),ethylene(ETH)treatment and NaCl salt stress,suggesting that the gene may be involved in hormone signal transduction pathway and stress response.This study provided theoretical basis for further exploration into the function and molecular mechanism of UDP-arabinopyranose mutase gene in plant response to stress.