柳属ARF家族的鉴定及生物信息学分析

Identification and Bioinformatics Analysis of ARF Family of Salix

ARF是一类存在于植物中的转录因子。通过与生长素应答基因启动子结合的方式,广泛作用于植物的生长发育,然而,柳属中的ARF家族成员的研究还未见报道。本研究根据拟南芥、番茄、水稻、毛果杨、杞柳、旱柳的基因组数据库和ARF蛋白的保守结构域对ARF家族成员进行了鉴定,在旱柳中鉴定出68个ARFs家族的成员并在6个物种间进行了进化分析,将ARF家族分为7个亚族,7个亚族内基因都很保守。对旱柳的68个ARF基因家族成员进一步进行保守域、基因结构、染色体定位等分析发现,旱柳ARF家族成员大部分具有B3、Auxin_resp两个结构域,从基因结构的角度来看,不同亚族彼此间差异很大,但各亚族内外显子保守性较高。编码ARF的68个基因被定位到了32个染色体上,在两个亚基因组上分布情况相似。进一步利用转录组数据对ARF家族成员在旱柳初生生长过程中的表达模式分析发现,SmARF12B、SmARF27等基因对于速生性状具有正调控的作用。而SmARF26、SmARF1B等基因表达可能对速生性存在一定负调控作用。本研究首次对柳属ARF家族进行生物信息学鉴定,并分析了其与速生性的关系,为深入研究旱柳中ARF蛋白家族的作用提供依据。

ARF is a kind of transcription factorexisting in plants.It plays an important role in plant growth and development by binding to the auxin response gene promoter.However,the research has not been reported about the members of the ARF family in Salix.In this study,the members of ARF family were identified based on the genomic databases of Arabidopsis thaliana,tomato,rice,Populus trichocarpa,Salix integra Linn.and Salix matsudana and the conserved domain of the protein of ARF.68 members of the ARF family were identified in Salix matsudana.And the evolutionary analysis was carried out among 6 species.The ARF family was divided into seven subgroups,and the genes in the seven subgroups were all conserved.We further analyzed the conserved domain,gene structure and chromosome location of 68 members of ARF gene family.The most of members of ARF family have two domains,B3and Auxin_resp in Salix matsudana.From the result of gene structure,there are great differences among different subgroups,but exons in each subgroup are highly conserved.68 genes encoding ARF were locted to 32 chromosomes.Furthermore,the expression pattern of the members of ARF family during the primary growth of Salix matsudana was analyzed by transcriptome data.Some genes have positive regulation on fast-growing traits,such as SmARF12B,SmARF27 and so on.While the expression of other genes may have negative regulation on fast-growing traits,such as SmARF26,SmARF1B and so on.In this study,the ARF family of Salix was identified by bioinformatics for the first time.We also analyzed the relationship between the family and fast-growing traits.These provide a basis for further study on the role of ARF protein family in Salix matsudana.