大豆Glyma08g11030启动子在拟南芥中的表达分析

Expression Analysis of Soybean Glyma08g11030 Promoter in Arabidopsis thaliana

F-box蛋白在调控植物对逆境胁迫应答过程中具有重要功能。为研究大豆F-box蛋白Glyma08g11030基因启动子的表达特性,利用冻融法将Glyma08g11030植物表达载体质粒Glyma08g11030-pro-pCAMBIA3301转入农杆菌GV3101菌株。通过农杆菌介导的浸花法将Glyma08g11030启动子转化野生型拟南芥,经过含10%草甘膦(Basta)除草剂筛选获得转Glyma08g11030启动子阳性植株。对纯合的T代转基因拟南芥进行GUS组织化学染色,结果表明Glyma08g11030启动子驱动的GUS基因在转基因拟南芥发育5和7 d的幼苗、莲座叶、茎生叶、茎和花序中均表达。研究结果为进一步利用Glyma08g11030启动子驱动目的基因表达提供参考。

F-box protein plays an important role in regulating the response of plants to stress.In order to study the expression characteristics of soybean F-box protein Glyma08g11030 gene promoter,the Glyma08g11030 plant expression vector plasmid Glyma08g11030-pro-pCAMBIA3301 was transformed into Agrobacterium strain GV3101 by freeze-thaw method.The Glyma08g11030 promoter was transformed into wild-type Arabidopsis thaliana by the Agrobacterium-mediated floral dip method,and the Glyma08g11030 promoter-positive plants were obtained after screening with 10% glyphosate(Basta) herbicide.GUS histochemical staining of homozygous Tgeneration transgenic Arabidopsis showed that the GUS gene driven by the Glyma08g11030 promoter was expressed in the 5 and 7 days seedlings,rosette leaves,stem leaves,stems and inflorescences of the transgenic Arabidopsis.The research results provide a reference for further using the Glyma08g11030 promoter to drive the expression of the target gene.