功能菌群DDMZ1固定化及其对RB5的处理效果研究

Immobilization of Functional Flora DDMZ1 and Its Treatment Effect on Dye Reactive Black 5

实验用聚乙烯醇(PVA)-海藻酸钠(SA)-沸石作为固定化复合载体,使用包埋交联法固定功能菌群DDMZ1,用正交实验对微生物固定化载体物理特性及偶氮染料活性黑5(RB5)降解脱色性能进行了分析和优化。实验结果发现:SA的浓度为1%、PVA的浓度为8%、沸石浓度为2%、菌液OD=0.7和交联时间12 h,包埋交联固定化条件最佳。扫描电镜结果显示:固定化PVA-SA-沸石载体小球内部结构孔隙众多,利于微生物细胞的附着和增殖。固定化功能菌群 DDMZ1 小球机械强度高、储存稳定性好,特别是它循环利用 30批次后,脱色率仍旧保持在90%以上。同时与游离菌相比,固定化功能菌群DDMZ1小球具有更宽的pH适应范围、更高的热稳定性以及更高盐度和染料浓度的耐受性,且具有循环使用次数越多耐受性越强的趋势。首次将天然功能菌群DDMZ1进行固定化并将其应用于偶氮染料活性黑5的处理,研究结果拓展了功能菌群DDMZ1的应用范围,同时对于利用固定化功能微生物处理含染料废水具有重要指导意义。

In this study, polyvinyl alcohol (PVA) -sodium alginate (SA) -zeolite was used as immobilized composite carrier,and the functional flora DDMZ1 was immobilized by embedding cross-linking method. The physical properties and decolorization performance of Reactive Black 5 (RB5) were analyzed and optimized by orthogonal experiment. The optimal conditions for preparation were 1% SA, 8% PVA, 2% zeolite, bacteria liquid OD=0.7 and crosslinking time 12 h. Scanning electron microscope results showed that the immobilized PVA-SA-zeolite carrier bead had numerous internal structure, which was conducive to the attachment and proliferation of microbial cells. The immobilized functional flora DDMZ1 pellets have high mechanical strength and good storage stability, especially after 30 batches of recycling, the decolorization rate still remains above 90%. Meanwhile, compared with the free flora, the immobilized functional flora DDMZ1 pellets had a wider range of pH adaptation, higher thermal stability, higher salinity and dye concentration tolerance, and had a trend of stronger tolerance with more cycles of use. DDMZ1 was immobilized for the first time and applied to the treatment of azo dye Reactive Black 5, The results expand the application range of functional flora DDMZ1, and have important guiding significance for the treatment of dye-containing wastewater by immobilized functional microorganisms.