慢性睡眠剥夺对小鼠糖、脂代谢的影响及网络药理机制研究

Effects of chronic sleep deprivation on glucose and lipid metabolism in mice and its network pharmacological mechanism

目的 本研究慢性睡眠剥夺(CSD)对小鼠糖脂代谢的影响及网络药理学机制。方法 实验中运用改良多平台水环境法建立CSD小鼠模型,通过监测小鼠空腹血糖(FBC),酶联免疫吸附试剂盒测定血清胰岛素、低密度脂蛋白(LDL)和高密度脂蛋白(HDL)水水平反映慢性睡眠剥夺对小鼠糖、脂代谢的影响。同时,利用网络药理学技术预测CSD影响小鼠糖脂代谢的作用机制。结果 与正常小鼠相比,睡眠剥夺CSD小鼠的FBG降低,胰岛素含量升高,胰岛素抵抗指数显著增加,血清LDL和HDL含量明显下降,睡眠剥夺小鼠出现糖脂代谢障碍和胰岛素抵抗。网络药理学分析结果显示,CSD可能通过影响PI3K-Akt信号通路,Rap1信号通路,Toll样受体信号通路等途径导致糖脂代谢紊乱。结论 CSD可降低小鼠血糖,诱导胰岛素抵抗,通过多种途径参与、诱发小鼠糖、脂代谢紊乱。

Objective To investigate the effect of chronic sleep deprivation(CSD) on glucose and lipid metabolism in mice and its network pharmacological mechanism.Methods The modified multi platform water environment method was used to establish the mouse model of CSD.The fasting blood glucose(FBG) of mice was monitored,and the levels of serum insulin,low-density lipoprotein LDL and high-density lipoprotein(HDL) were measured by enzyme-linked immunosorbent assay kit to reflect the effect of CSD on glucose and lipid metabolism in mice.Meanwhile,network pharmacology technology was used to predict the mechanism of CSD affecting glucose and lipid metabolism in mice.Results Compared with normal mice,FBG decreased,insulin content increased,insulin resistance index increased significantly,serum LDL and HDL decreased significantly,and glucose and lipid metabolism disorder and insulin resistance appeared in sleep deprived mice.The results of network pharmacological analysis show that chronic sleep deprivation may lead to the disorder of glucose and lipid metabolism by affecting PI3 K Akt signal pathway,Rapl signal pathway and Toll like receptor signal pathway.Conclusion CSD can reduce blood glucose and induce insulin resistance in mice,and lead to the disorder of glucose and lipid metabolism in mice.