HIF-1α、VEGF在口腔癌组织中的表达水平及其临床意义

Expression and Clinical Significance of HIF-1αand VEGF in Oral Cancer Tissues

【目的】探讨低氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)在口腔癌组织中的表达水平及其临床意义。【方法】选择2014年5月至2016年2月在陕西省榆林市第四医院拟行根治性手术治疗的174例口腔癌患者,在术中分别采集癌组织与癌旁组织(距离肿瘤组织边缘>3 cm)。采用免疫组化法检测口腔癌患者癌组织与癌旁组织中HIF-1α与VEGF的表达情况,分析口腔癌患者癌组织中HIF-1α与VEGF表达水平与临床病理特征的关系。随访5年,采用Kaplan-Meier法绘制生存曲线,并采用Log-rank检验HIF-1α阳性与HIF-1α阴性患者及VEGF阳性与VEGF阴性患者生存曲线的差异。【结果】口腔癌患者癌组织中HIF-1α、VEGF阳性率均显著高于癌旁组织(P<0.05);口腔癌患者癌组织中HIF-1α与VEGF水平与淋巴结是否转移、肿瘤直径、临床分期、肿瘤分化程度有关(P<0.05),而与性别、年龄、血管是否侵犯、神经是否侵犯、发病部位无关(P>0.05)。HIF-1α阴性患者5年生存率显著高于HIF-1α阳性患者(P<0.05);HIF-1α阴性患者的中位生存时间为42.63个月,显著长于HIF-1α阳性患者的25.01个月(P<0.05);VEGF阴性患者5年生存率显著高于VEGF阳性患者(P<0.05);VEGF阴性患者的中位生存时间为45.84个月,显著高于VEGF阳性患者的16.94个月(P<0.05)。【结论】口腔癌患者癌组织中HIF-1α、VEGF阳性率较高,与患者淋巴结是否转移、肿瘤直径、临床分期、肿瘤分化程度有关,且检测二者表达水平有助于预测患者预后。

【Objective】To investigate the expression and clinical significance of hypoxia inducible factor-1α(HIF-1α)and vascular endothelial growth factor(VEGF)in oral cancer tissues.【Methods】A total of 174 patients with oral cancer who were treated by the Fourth Hospital of Yulin City,Shaanxi Province from May 2014 to February 2016 were selected as the research objects.During the operation,adjacent tissues(>3 cm from the edge of tumor tissue)and cancer tissues were collected.Immunohistochemical method was used to detect the expression of HIF-1α and VEGF in cancer tissues and adjacent tissues of oral cancer patients.General patient data were collected.The relationship of HIF-1α and VEGF expression levels in the cancer tissues of oral cancer patients with the clinicopathological characteristics were analyzed.After 5 years of follow-up,Kaplan-Meier method was used to draw the survival curve,and the Log-rank test was used to test the difference in survival curves of HIF-1α positive vs HIF-1α negative patients,and VEGF positive vs VEGF negative patients.【Results】The positive rates of HIF-1α and VEGF in cancer tissues of oral cancer patients were higher than those in adjacent tissues(P<0.05).The expression of HIF-1α and VEGF in the cancer tissues of oral cancer patients were related to lymph node metastasis,tumor size,clinical stage,and tumor differentiation(P<0.05),but not related to gender,age,blood vessel invasion,nerve invasion,and location of disease(P>0.05).The 5-year survival rate of HIF-1α-negative patients is higher than that of HIF-1α-positive patients(P<0.05).The median survival time of HIF-1α-negative was 42.63 months,which was longer than that of HIF-1α-positive patients(25.01 months).There was a statistically significant difference between the survival curves of the two population(P<0.05).The 5-year survival rate of VEGF-negative patients was higher than that of VEGF-positive patients(P<0.05).The median survival time of VEGF-negative and VEGF-positive patients was 45.84 months and 16.94 months,respectively,and the difference in survival curves was statistically significant(P<0.05).【Conclusion】The positive rates of HIF-1α and VEGF in the cancer tissues of patients with oral cancer are relatively higher,which are related to lymph node metastasis,tumor size,clinical stage,and degree of tumor differentiation.The detection of both HIF-1α and VEGF expression can help predict the prognosis in oral cancer patients.