MeCP2和TNC在子宫肌瘤组织中的表达水平及其临床意义

Expression Level of MeCP2 and TNC in Uterinemyoma and its Clinical Significance

【目的】探讨甲基化CpG结合蛋白2(MeCP2)和肌腱蛋白C(TNC)在子宫肌瘤组织中的表达水平及其临床意义。【方法】选取2019年1月至2021年9月陕西省康复医院收治的109例子宫肌瘤患者为观察组,另选取68例同期因子宫脱垂行子宫切除术患者为对照组。收集两组患者基本资料,采取免疫组化法检测观察组子宫肌瘤组织、子宫肌瘤旁组织及对照组子宫平滑肌组织中MeCP2和TNC蛋白表达情况。分析子宫肌瘤患者子宫肌瘤组织及瘤旁组织中MeCP2和TNC蛋白表达情况,分析子宫肌瘤发生的影响因素,分析观察组MeCP2和TNC水平与子宫肌瘤直径关系。【结果】子宫肌瘤组织中MeCP2、TNC阳性表达率均高于子宫肌瘤旁组织(P<0.05)。Logistic多因素回归分析结果显示月经紊乱(OR:3.892,95%CI:1.576~9.611)、流产次数(OR:4.293,95%CI:1.739~10.601)、MeCP2阳性(OR:4.495,95%CI:2.218~9.110)、TNC阳性表达(OR:4.341,95%CI:2.142~8.796)是子宫肌瘤发生的影响因素(P<0.05)。子宫肌瘤≥5 cm患者MeCP2、TNC阳性表达率均高于子宫肌瘤<5 cm患者(P<0.05)。【结论】子宫肌瘤发生与子宫平滑肌组织中MeCP2、TNC表达有关,且其表达水平可能与子宫肌瘤直径相关。

【Objective】To investigate the expression level of methylated CpG binding protein 2(MeCP2)and tenascin C(TNC)in uterinemyoma and its clinical significance.【Methods】A total of 109 patients with hysteromyoma treated in the Shaanxi Rehabilitation Hospital from January 2019 to September 2021 were selected as the observation group,and 68 patients who underwent hysterectomy for uterine prolapse in the same period were selected as the control group.The basic data of the two groups of patients were collected,and the expression of MeCP2 and TNC protein in uterinemyoma tissue,adjacent tissue of uterinemyoma in the observation group and uterine smooth muscle tissue in the control group were detected by immunohistochemical method.The expression of MeCP2 and TNC protein in uterine leiomyoma tissue and adjacent tissue of patients with uterine leiomyoma was analyzed,and the influencing factors of uterine leiomyoma were analyzed.At the same time,the relationship between MeCP2 and TNC levels and uterine leiomyoma diameter in the observation group was also analyzed.【Results】The positive expression rates of MeCP2 and TNC in uterine leiomyoma were higher than those in adjacent tissues(P<0.05).Logistic regression analysis showed that menstrual disorder(OR:3.892,95%CI:1.576~9.611),the number of abortions(OR:4.293,95%CI:1.739~10.601),MeCP2 positive(OR:4.495,95%CI:2.218~9.110),TNC positive expression(OR:4.341,95%CI:2.142~8.796)were the influencing factors of hysteromyoma(P<0.05).The positive expression rates of MeCP2 and TNC in patients with hysteromyoma≥5 cm were higher than those in patients with hysteromyoma<5 cm(P<0.05).【Conclusion】The occurrence of uterinemyoma is related to the expression of MeCP2 and TNC in uterine smooth muscle tissue,and its expression level may be related to the diameter of uterinemyoma.