中药神农仕复方制剂对猪繁殖与呼吸综合征防治效果研究

Prevention and control effect of traditional Chinese medicine on porcine reproductive and respiratory syndrome virus

为探索中药神农仕复方制剂对猪繁殖与呼吸综合征(porcinereproductiveandrespiratory syndrome,PRRS)的防治效果,本研究选取28日龄健康断奶仔猪40头,随机分为4组,10头/组,分别为空白对照组(negative control group)、攻毒对照组(positive control group)及中药1组(2 kg/t)、中药2组(4 kg/t)组,其中中药1和中药2组分别于攻毒前7 d饲喂中药,攻毒后连续饲喂14 d,每日观察临床症状及测定直肠体温,定期体重测量,试验结束进行病理剖检与肺脏病理组织切片观察、评分。结果显示,相比空白对照组,连续7 d添加中药(2 kg/t、4 kg/t)后可显著提高仔猪日增重(P<0.05);相比攻毒对照组的存活率为80%,中药组可显著提高仔猪存活率至100%(P<0.01),且于攻毒后8 d临床症状减轻,体温恢复正常,肺脏未见明显病变,但可见肺泡间隔增厚并伴有轻度炎性细胞浸润,与攻毒对照组差异不显著(P>0.05);综上研究表明,中药神农仕复方制剂可显著提高日增重,有效改善PRRS病毒(PRRSV)感染猪的临床症状及肺部病理变化。本研究为中药防治PRRS提供了试验依据。

In order to investigate on the therapeutic effect of Shennongshi Chinese medicine powder on PRRS, forty 4-week-old pigs were randomly divided into four groups: negative control group, positive control group, Chinese medicine prescription group 1(2 kg/t), Chinese medicine prescription group 2(4 kg/t). Groups 1 and 2 each were added with herb 7 days before PRRSV challenge, and were continuous fed for 14 days. The clinical symptoms and rectal temperature were observed every day,and the body weight was measured regularly. At the end of the experiment, pathological autopsy and lung samples were collected for pathological tissue section observation and score. The results showed that compared with the negative control group, adding traditional Chinese medicine(2 kg/t, 4 kg/t)for 7 days could significantly increase the daily gain of piglets(P<0.05);The survival rate of piglets in the traditional Chinese medicine experimental group could be significantly improved to 100%(P<0.01)when compared with the negative control group(80%);After 8 days of challenge, the clinical symptoms were relieved, the body temperature returned to normal, there was no obvious lesion in the lung, but there was thickening of alveolar septum with mild inflammatory cell infiltration, which was not significantly different from the control group(P>0.05). In conclusion, this study shows that Shennongshi Chinese medicine powder can significantly improve daily weight gain and effectively improve the clinical symptoms and lung pathological changes of PRRSV infected pigs. This study provides an experimental basis for the prevention and treatment of PRRS with traditional Chinese medicine.