平腑调代方对肥胖2型糖尿病小鼠脂代谢和炎症及氧化应激水平的影响

Effects of Pingfutiaodai decoction on lipid metabolism, inflammation and oxidative stress in obese type 2 diabetic mice

目的观察平腑调代方对肥胖2型糖尿病小鼠脂代谢、炎症及氧化应激水平的影响。方法将36只无特定病原体级健康雄性6周龄C57BL/6J小鼠依据随机数字表法分为对照组、模型组和中药组,模型组选用高脂饲料联合链脲佐菌素诱导建立肥胖2型糖尿病小鼠模型,并给予纯水灌胃干预,中药组诱导建立肥胖2型糖尿病小鼠模型并给予平腑调代方灌胃干预。对照组不诱导肥胖2型糖尿病模型并给予纯水灌胃干预。干预12周后,检测各组总胆固醇、三酰甘油、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、C反应蛋白(CRP)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)、超氧化物歧化酶(SOD)、丙二醛、总抗氧化能力(T-AOC)水平。结果 造模结束后,剔除不达标小鼠,最终对照组10只小鼠,模型组与中药组各9只小鼠进行药物干预。干预12周后,模型组总胆固醇、三酰甘油、LDL-C水平高于对照组,HDL-C水平低于对照组,中药组总胆固醇、三酰甘油、LDL-C水平均低于模型组,HDL-C水平高于模型组(均P<0.05)。模型组CRP、IL-6及TNF-α水平明显高于对照组,中药组CRP、IL-6、TNF-α水平低于模型组[(48±5)μg/L比(55±6)μg/L、(66.1±5.8)ng/L比(71.9±2.5)ng/L、(97±9)ng/L比(126±11)ng/L](均P<0.05)。模型组SOD、T-AOC水平低于对照组,丙二醛水平高于对照组,中药组SOD、T-AOC水平高于模型组,丙二醛水平低于模型组(均P<0.05)。结论平腑调代方能够改善肥胖2型糖尿病小鼠脂代谢紊乱,减轻炎症反应并提高抗氧化能力,对肥胖2型糖尿病的治疗有参考意义。

Objective To observe the effects of Pingfutiaodai decoction on lipid metabolism, inflammation and oxidative stress in obese type 2 diabetic mice. Methods Totally 36 healthy male C57 BL/6 J mice without specific pathogens at the age of 6 weeks were divided into control group, model group and traditional Chinese medicine group according to random number table method. The model group was induced to establish obese type 2 diabetic mice model by high-fat diet combined with streptozotocin and was given pure water gavage intervention. The traditional Chinese medicine group was induced to establish obese type 2 diabetic mice model and was given Pingfutiaodai decoction gavage intervention. The control group was not induced to obese type 2 diabetic model and was given pure water gavage intervention. After 12 weeks of intervention, levels of total cholesterol, triacylglycerol, high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), C-reactive protein(CRP), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α), superoxide dismutase(SOD), malondialdehyde and total-antioxidant capacity(T-AOC) were measured in each group. Results After modeling and removing non-compliance mice, 10 mice in the control group, 9 mice in the model group and 9 mice in the traditional Chinese medicine group were given drug intervention. Twelve weeks after intervention, the levels of total cholesterol, triglyceride and LDL-C in the model group were higher than those in the control group, and the level of HDL-C was lower than that in the control group;the levels of total cholesterol, triglyceride and LDL-C in the traditional Chinese medicine group were lower than those in the model group, and the level of HDL-C was higher than that in the model group(all P<0.05). The levels of CRP, IL-6 and TNF-α in the model group were higher than those in the control group, and the levels in the traditional Chinese medicine group were lower than those in the model group[(48±5)μg/L vs(55±6)μg/L,(66.1±5.8)ng/L vs(71.9±2.5)ng/L,(97±9)ng/L vs(126±11)ng/L](all P<0.05). The levels of SOD and T-AOC in the model group were lower than those in the control group, and the level of malondialdehyde was higher than that in the control group;the levels of SOD and T-AOC in the traditional Chinese medicine group were higher than those in the model group, and the level of malondialdehyde was lower than that in the model group(all P<0.05). Conclusion Pingfutiaodai decoction can improve the disorder of lipid metabolism, reduce inflammatory response and increase antioxidant capacity in obese type 2 diabetic mice, which has reference significance for the treatment of obese type 2 diabetes mellitus.