MFCs降解布洛芬性能及电极生物膜宏基因组分析

Ibuprofen degradation in MFCs and metagenomics analysis of electrode biofilms

布洛芬作为一种高性价比消炎药被大量使用,环境中布洛芬浓度升高易引起抗生素抗性基因污染等环境问题。本研究构建了双室微生物燃料电池(MFCs)以去除水中布洛芬,分析了阳极生物膜微生物群落结构并注释了基因功能。结果表明,MFCs阳极微生物以10 mg·L^(-1)布洛芬作为唯一碳源,外接1000Ω电阻,输出电压约为0.60 V,运行至108 h时布洛芬去除率达85.33%,是对照厌氧生物处理的3.18倍。微生物群落结构分析结果表明,MFCs阳极生物膜微生物群落结构与传统厌氧生物膜差异显著,Proteobacteria在阳极生物膜相对丰度高达83.57%,而对照组中仅为60.52%,Pusillimonas、Burkholderia、Agrobacterium、Martelella及Ruegeria在属水平相对丰度也高于对照组。代谢通路分析结果表明,环境信息处理通路在MFCs阳极微生物显著增强,其碳代谢及氮代谢基因数量分别高于对照组9.02%和28.58%。

Ibuprofen is a widely used non-steroidal anti-inflammatory drug with high performance and low price.The increase of ibuprofen concentration in the environment is easy to cause environmental problems such as resistance gene contamination.In this study,dual-chamber microbial fuel cells(MFCs)were constructed to remove ibuprofen from water,and metagenomic sequencing was used to analyze the microbial community structures and annotate functional genes of anodic biofilm.The results showed that the maximum voltage was about 0.60 V generated from MFCs when anodic microbes took ibuprofen as the sole carbon source and the external resistance was 1000Ω,and ibuprofen removal efficiency reached to 85.33%at 108h of MFCs running,which was 3.18 times of the anaerobic control group.Compared with the traditional anaerobic method,the composition of the microbial community in the anodic biofilm also changed significantly.At phyla level,the relative abundance of Proteobacteria was 83.57%in anodic biofilm,which was just 60.52%in control group.At genus level,the relative abundance of Pusillimonas,Burkholderia,Agrobacterium,Martelella and Ruegeria was also higher than that in control group.Metabolic pathway analysis showed that the environmental information processing pathway was significantly enhanced in anodic biofilm microorganisms,and the genes related to carbon and nitrogen metabolism were 9.02%and 28.58%higher than those of control group,respectively.