PIN1基因rs2233679位点多态性与女性HPV感染宫颈癌易感性的关联

Association between PIN1 gene RS2233679 site polymorphism and female susceptibility to HPV infection with cervical cancer

目的 探讨肽脯氨酰基顺反异构酶1(PIN1)基因rs2233679位点多态性与女性HPV感染宫颈癌易感性的关系。方法 选择青岛市中医医院妇科2018年4月-2020年10月进行治疗的HPV阳性新发宫颈癌女性70例为研究组,选择同期医院进行妇科检查HPV-DNA筛查结果阳性的健康女性70名为对照组。研究对象均在入组时抽取外周静脉血,提取全血DNA后,采用限制性片段长度多态性聚合酶链反应检测PIN1基因rs2233679位点基因分型。结果 哈迪-温伯格平衡检验结果显示,研究组与对照组对象理论值与实际值基因型频率分布比较差异无统计学意义,说明该群体具有一定的群体代表性;研究组PIN1基因rs2233679位点TT基因型频率(35.71%)高于对照组(P<0.05);校正研究对象年龄、孕次、产次、BMI、HPV型别等常量参数后,携带TT基因型为HPV感染后发生宫颈癌的独立危险因素(P<0.05);PIN1基因rs2233679位点TT型宫颈癌患者与CT/CC型患者肿瘤分期比较差异有统计学意义(P<0.05)。结论 PIN1基因rs2233679位点TT基因型与HPV感染后发展为宫颈癌的风险增加有关,同时该基因型也增加病情进展风险。

OBJECTIVE To investigate the relationship between Peptide Prolyl Cis-Trans Isomerase(PIN1) Gene RS2233679 site polymorphism and female susceptibility to HPV in patients with cervical cancer. METHODS Total of 70 HPV-positive women with new cervical cancer who were treated in the Department of Gynecology of Qingdao Hospital of Traditional Chinese Medicine from Apr 2018 to Oct 2020 were enrolled in the study group. And 70 healthy women with HPV-positive under gynecological examination during the same period in the hospital were in the control group. Peripheral venous blood was taken from all subjects at enrollment, and whole blood DNA was extracted, and genotype of PIN1 gene rs2233679 was detected by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism(PCR-RFLP). RESULTS Hardy-Winberg Balance Inspection showed that differences between theoretical value and the actual value of genotype frequency distribution in the research group and the control group were not significant, indicating a certain group representation. The genotype frequency of TT at PIN1 Gene rs2233679 site(35.71%) was significantly higher than that in the control group(P<0.05). After being adjusted by calibrating constant parameters such as pregnancy, primary, bmi, HPV type, and etc., analysis showed that carrying TT genotype was the independent risk factors for cervical cancer after HPV infection(P<0.05). There were significant differences in tumor staging between TT cervical cancer patients at RS2233679 of PIN1 gene and CT/CC patients(P<0.05). CONCLUSION TT genotype at Pin1 gene rs2233679 site is related to the risk of cervical cancer after HPV infection, and also increases the progression of the disease.