黄芩苷对SAP并发胰周感染大鼠T淋巴细胞亚群及相关细胞因子水平的影响

Effect of Baicalin on T lymphocyte subsets and related cytokine levels in rats with SAP complicated with peripancreatic infection

目的探讨黄芩苷对重症急性胰腺炎(SAP)并发胰周感染模型大鼠的保护作用及机制。方法采用胰腺管注射5%牛磺胆酸钠、脂多糖(LPS)制备SAP并发胰周感染模型大鼠;观察实验组[对照组(DZ)、低剂量组(L)、中剂量组(M)、高剂量组(H)]和空白组(CK)大鼠健康状况、胰脏组织学指标、炎症细胞数量、腹腔灌洗液菌落数,测定白细胞介素-1β(IL-1β)、IL-12、IL-17A、转化生长因子-β1(TGF-β1)蛋白表达水平,IL-1β、IL-12、IL-17A、TGF-β1 mRNA水平,T淋巴细胞亚群水平。结果模型组大鼠有发热现象,体温、体质量、外周血炎症细胞分类计数组间差异有统计学意义,对照组腹腔灌洗液菌落计数高,黄芩苷治疗组计数低(P<0.05);SAP大鼠胰脏IL-1β、TGF-β1蛋白表达水平高于CK组,IL-12的表达低于CK组(P<0.05),IL-17A mRNA、TGF-β1mRNA差异有统计学意义(P<0.05);CK、DZ、L、M、H组大鼠CD_(3)^(+)、CD_(4)^(+)、CD_(4)^(+)/CD_(8)^(+)上升,CD_(8)^(+)比例下降,SAP组大鼠CD_(3)^(+)、CD_(4)^(+)、CD_(4)^(+)/CD_(8)^(+)升高。结论黄芩苷可通过介导SAP并发胰周感染大鼠体内T淋巴细胞亚群转化比例,调整相关细胞因子水平,发挥正向免疫调节的作用。

OBJECTIVE To investigate the protective effect and mechanism of Baicalin on rats with SAP complicated with peripancreatic infection.METHODS The rats model of SAP complicated with peripancreatic infection was established by injecting 5%sodium taurocholate and LPS into the pancreatic duct.The health status,pancreatic histological indicators,inflammatory cell number,and number of colonies in the peritoneal lavage fluid of the rats in each experimental group[the control group(DZ),the low dose group(L),the medium dose group(M),the high dose group(H),the blank group(CK)]were observed.The protein expression of IL-1β,IL-12,IL-17 A,and TGF-β1,the mRNA levels of IL-1β,IL-12,IL-17 A,and TGF-β1,and the levels of T lymphocyte subsets were determined.RESULTS The rats in the model group had fever,and there were significant differences in body temperature,body weight,and inflammatory cell counts in the peripheral blood among groups.The colony counts in the peritoneal lavage fluid of the control group were higher,and the baicalin treatment group had significantly lower counts(P<0.05);The expression levels of IL-1β,and TGF-β1 proteins in pancreas of SAP rats were significantly higher than those in the CK group,while the expression of IL-12 was significantly lower than those in the CK group(P<0.05).The differences in IL-17 A mRNA and TGF-β1 mRNA were significant(P<0.0,5);The CD_(3)^(+),CD_(4)^(+),CD_(4)^(+)/CD_(8)^(+) ratio of rats in CK,DZ,L,M,and H groups increased,and the ratio of CD_(8)^(+)decreased,while the CD_(3)^(+),CD_(4)^(+),CD_(4)^(+)/CD_(8)^(+) of rats in SAP group increased.CONCLUSION Baicalin mediated the transformation ratio of T lymphocyte subsets in rats with SAP complicated by peripancreatic infection,adjusted the levels of related cytokines,and played a positive immunomodulatory effect.