Hp感染与胃癌及胃癌前病变组织MUC2、MUC5AC、MUC6、CD10表达的关系

Relationship between Hp infection and expression of MUC2,MUC5AC,MUC6and CD10in gastric cancer and precancerous lesion tissues

目的 探究幽门螺杆菌(Hp)感染与胃癌及胃癌前病变组织黏蛋白(MUC)2、MUC5AC、MUC6、CD10表达的关系。方法 选择2018年6月-2020年6月青海省人民医院收治的209例胃癌患者(胃癌组)和184例胃癌癌前病变患者(胃癌前病变组),采用快速尿素酶试验(RUT)和改良 Giemsa染色法检测 Hp感染情况,收集胃病变组织标本,采用免疫组化法检测 MUC2、MUC5AC、MUC6、CD10蛋白表达情况,比较两组 Hp感染阳性率及MUC2、MUC5AC、MUC6、CD10蛋白表达水平,并分析 MUC2、MUC5AC、MUC6、CD10表达与 Hp感染的关系。结果 胃癌组 Hp感染阳性率为69.38%(145/209),高于胃癌前病变组的54.35%(100/184)(P<0.05);免疫组化结果显示,胃癌组 MUC2、CD10表达阳性率高于胃癌前病变组(P<0.05),MUC5AC、MUC6表达阳性率低于胃癌前病变组(P<0.05);Hp感染阳性胃癌前病变及胃癌病灶组织中 MUC2、CD10表达阳性率高于 Hp感染阴性(P<0.05),Hp感染阳性胃癌前病变及胃癌病灶组织中 MUC5AC、MUC6表达阳性率低于 Hp感染阴性(P<0.05)。结论 Hp感染可促进胃癌 MUC2、CD10表达和抑制 MUC5AC、MUC6表达,MUC2、CD10上调表达与MUC5AC、MUC6下调表达以及 Hp感染均为胃癌发生和发展的重要因素。

OBJECTIVE To analyze the relationship between Helicobacter pylori (Hp)infection and expression of mucin(MUC)2,MUC5AC,MUC6and CD10in gastric cancer and precancerous lesion tissues.METHODS Totally 209gastric cancer patients(the gastric cancer group)and 184patients with gastric precancerous lesions(the precancerous lesion group)who were treated in Qinghai Provincial People’s Hospital from Jun 2018to Jun 2020were enrolled in the study.The Hp infection was detected by rapid urease test(RUT)and modified Giemsa staining.The gastric lesion tissue specimens were collected,the positive rates of expression of MUC2,MUC5AC,MUC6and CD10were detected by immunohistochemistry.The positive rate of Hp infection and expression levels of MUC2,MUC5AC,MUC6and CD10were compared between the two groups,and the relationship between the expression of MUC2,MUC5AC,MUC6and CD10and the Hp infection was observed.RESULTS The positive rate of Hp infection of the gastric cancer group was 69.38%(145/209),significantly higher than 54.35%(100/184)of the precancerous lesion group(P<0.05).The result of immunohistochemistry assay showed that the positive rate of expression of MUC2and CD10of the gastric cancer group were significantly higher than those of the precancerous lesion group(P<0.05),while the positive rate of expression of MUC5AC and MUC6of the gastric cancer group were significantly lower than those of the precancerous lesion group(P<0.05).The positive rates of expression of MUC2and CD10in gastric precancerous lesion tissues and gastric cancer lesion tissues were significantly higher among the Hp-positive patients than among the Hp-negative patients(P<0.05),while the positive rates of expression of MUC5AC and MUC6in gastric precancerous lesion tissues and gastric cancer lesion tissues were significantly lower among the Hp-positive patients than among the Hp-negative patients (P <0.05).CONCLUSIONThe Hp infection can promote the expressions of MUC2and CD10and inhibit the expressions of MUC5AC and MUC6in gastric cancer.The up-regulated expressions of MUC2and CD10and the down-regulated expressions of MUC5AC and MUC6as well as Hp infection are important factors for the occurrence and development of gastric cancer.