红叶石楠离体组培快繁技术体系研究

Technical System of Rapid Propagation of Tissue Culture in vitro of Photinia fraseri

以红叶石楠最常见品种“红罗宾”为试材,采用截取其带芽茎段及带顶芽段为外植体进行植物组织培养的方法,研究了初代培养中不同培养基激素组合对愈伤组织的影响、增殖培养中不同培养基激素组合对不定芽的影响和生根培养中不同培养基激素组合对生根的影响,筛选出红叶石楠离体快繁组培过程中不同阶段培养基激素浓度最佳组合,以期为红叶石楠规模化育苗提供参考依据。结果表明:初代培养的最适培养基激素组合为MS+0.4mg·L^(-1) BA+0.1mg·L^(-1) IBA;按照正交实验设计,增殖培养的最佳增殖培养基植物激素组合为MS+1.6mg·L^(-1) BA+0.4mg·L^(-1) KT+0mg·L^(-1) NAA(即不添加NAA)+0.2mg·L^(-1) IBA;生根培养基最佳激素组合为1/2MS+0.2mg·L^(-1) IBA。

The most common Photinia fraseri‘Red Robin’were used as experiment materials,this experiment was carried out by cutting the stem segments with buds and the top bud segments as explants to cultivate tissue.While it focused on the effects of different hormone combinations on calli in primary culture,of different hormone combinations on adventitious buds in proliferation culture and of different hormone combinations on rooting in rooting culture,in order to screen out the best combination of hormone concentration in different stages of tissue culture of Photinia fraseri in vitro and provide reference for large-scale breeding of Photinia fraseri.The results showed that the most suitable medium for the first generation culture of Photinia fraseri was MS+0.4 mg·L^(-1) BA+0.1mg·L^(-1) IBA.According to the orthogonal design experiment,the optimal phytohormone combination of medium for proliferation culture of Photinia fraseri was MS+1.6 mg·L^(-1) BA+0.4mg·L^(-1) KT+0mg·L^(-1) NAA(That was,no NAA)+0.2mg·L^(-1) IBA.The optimal medium for rooting culture of Photinia fraseri was 1/2MS+0.2mg·L^(-1) IBA.