陆地棉CPP转录因子家族全基因组鉴定及分析

Genome-wide Identification and Analysis of CPP Transcription Factor Family in Gossypium hirsutum L.

CPP(Cystein-rich polycomb-like protein or Tesmin/TOS1-like)家族是一类含有保守的富含半胱氨酸的CRC结构域的转录因子,在调控植物生长发育和非生物胁迫耐受过程中有重要作用。本研究通过对陆地棉全基因组的鉴定,获得20个GhCPP转录因子,均具有典型的CXC结构域。将拟南芥、水稻以及陆地棉的CPP家族成员进行系统进化分析,结果表明CPP基因家族分为两个大类(A和B)和四个亚类(Ⅰ~Ⅳ),GhCPP家族成员与拟南芥在进化关系上更为接近。进一步分析发现A大类与B大类GhCPP基因结构以及逆境响应表达模式上存在明显差异,具体表现为:A大类Gh CPP基因的CRC结构域均分布在蛋白序列的N端,而B大类除GhCPP12外,CRC结构域均分布在蛋白序列的C端;A大类和B大类GhCPP基因的外显子个数分别为7~9和9~12;表达量聚类分析表明,在高温、低温、高盐及干旱胁迫下,A大类的表达量均会表现下调,而大部分的B大类GhCPP基因在高温处理12 h和低温处理6 h时出现表达量上调。研究结果为进一步研究陆地棉GhCPP转录因子家族的功能提供理论基础。

The CPP(cyste-Rich polycomb-like protein or Tesmin/TOS1-like)family is a class of transcription factors containing conserved Cystein-rich CRC domains,which play an important role in regulating plant growth and development and abiotic stress resistance.In this study,genome-wide identification of GhCPP gene family members in Gossypium hirsutum L.was performed and revealed 20 CPP transcription factors containingtypical CXC domains.Phylogenetic analysis of CPP transcription factors from Arabidopsis thaliana,Oryza sativa L.and Gossypium hirsutum L.showed a more close relationship between members in Arabidopsis thaliana and Gossypium hirsutum L.and revealed that GhCPP family members are divided into two groups(A and B)and four subgroups(Ⅰ~Ⅳ)further analysis revealed that GhCPP genes of type A and type B were significantly different in gene structure and expression pattern responsive to abiotic stresses.To sum up,the CRC domains of GhCPP gene of type A were all distributed at the N-terminal of the protein sequence,while the CRC domains of type B were all distributed at the C-terminal of the protein sequence except GhCPP12.The number of exons of GhCPP gene in type A and type B was 7~9 and 9~12,respectively,with significant difference in number.Cluster analysis of expression pattern of A and B type GhCPP genes indicated all A type GhCPPs gene expression was repressed under cold,salt and drought stress while expression of most B type members was sharply induced under high temperature treatment for 12 h or under low temperature for 6 h.The results lay the foundation for further study on the function of GhCPP transcription factors in upland cotton.