非特异性阴道炎患者血清及阴道灌洗液细胞因子水平分析

Analysis of cytokine levels in serum and vaginal lavage fluid of patients with nonspecific vaginitis

目的探讨非特异性阴道炎患者血清及阴道灌洗液白介素-2(IL-2)、IL-8、IL-10和肿瘤坏死因子(TNF-α)水平变化及临床意义。方法选取2020年6月至2021年6月在许昌市建安区妇幼保健院治疗的108例非特异性阴道炎患者为研究对象(研究组),另选取同期本院体检健康的志愿者68例为对照组,研究组患者给予药物治疗,检测比较两组血清及阴道灌洗液IL-2、IL-8、IL-10和TNF-α水平变化。结果108例患者共检出120株病原菌,且对病原菌分析发现,链球菌属感染最为常见,占比36.67%,葡萄球菌属感染次之,占比23.33%;治疗前研究组血清及阴道灌洗液中IL-2、TNF-α低于对照组,IL-8、IL-10、IL-6高于对照组,差异有统计学意义(P<0.05),且研究组治疗后血清及阴道灌洗液中IL-2、TNF-α均显著升高,IL-8、IL-10、IL-6降低,差异有统计学意义(P<0.05),但研究组治疗后血清及阴道灌洗液中以上指标与对照组比较,差异无统计学意义(P>0.05);治疗前研究组血清及阴道灌洗液中CD3^(+)、CD4^(+)、CD8^(+)显著低于对照组,差异有统计学意义(P<0.05),且研究组治疗后血清及阴道灌洗液中CD3^(+)、CD4^(+)、CD8^(+)均显著升高,差异有统计学意义(P<0.05),但研究组治疗后血清及阴道灌洗液中以上指标与对照组比较,差异无统计学意义(P>0.05);相关性分析发现,血清及阴道灌洗液中炎症因子与T细胞群指标无相关性(P>0.05)。结论非特异性阴道炎患者血清及阴道灌洗液中IL-2、TNF-α、CD3^(+)、CD4^(+)、CD8^(+)呈低表达,IL-8、IL-10、IL-6呈高表达,说明以上因子失衡可能是介导非特异性阴道炎患者免疫功能失衡,促进疾病进展的重要因素。

Objective To explore the changes of interleukin-2(IL-2),IL-8,IL-10 and tumor necrosis factorα(TNF-α)levels in serum and vaginal lavage fluid of patients with non-specific vaginitis.Methods 108 patients with non-specific vaginitis treated in Maternal and Child Health Hospital of Jian'an District,Xuchang City between June 2020 and June 2021 were selected as the study group.Meanwhile,68 healthy volunteers were selected as the control group.Patients in the study group were given drug treatment.The levels of IL-2,IL-8,IL-10 and TNF-α in serum and vaginal lavage fluid were compared between the two groups.Results A total of 120 pathogenic bacteria strains were detected in the 108 patients,and the proportion of Streptococcus was the highest(36.67%),followed by Staphylococcus(23.33%).Before treatment,the levels of IL-2 and TNF-αin serum and vaginal lavage fluid in the study group were lower than those in the control group,while the levels of IL-8,IL-10 and IL-6 were higher than those in the control group,the difference was statistically significant(P<0.05).After treatment,the levels of IL-2 and TNF-α in serum and vaginal lavage fluid were significantly increased,while the levels of IL-8,IL-10 and IL-6 were decreased in the study group(P<0.05),but the difference in the above indicators between the two groups was not statistically significant(P>0.05).Before treatment,the levels of CD3^(+),CD4^(+) and CD8^(+) in serum and vaginal lavage fluid in the study group were significantly lower than those in the control group(P<0.05),which were significantly increased in the study group after treatment(P<0.05).The differences in the above indicators between the two groups were not statistically significant(P>0.05).Correlation analysis showed that there was no correlation between inflammatory factors in serum and vaginal lavage fluid and T cell subsets(P>0.05).Conclusion The serum and vaginal lavage fluid of patients with non-specific vaginitis showed low expression of IL-2,TNF-α,CD3^(+),CD4^(+),and CD8^(+),and high expression of IL-8,IL-10,and IL-6,indicating that the imbalance of the above factors may be caused by an important factor that mediates the imbalance of immune function in patients with nonspecific vaginitis and promotes disease progression.