广西地区患者ABO亚型血清学与分子生物学研究

A study on serology and molecular biology of ABO subtypes in patients in Guangxi Zhuang Autonomous Region

目的调查广西地区患者ABO亚型血清学和分子生物学特征。方法选取2021年广西地区患者经血型血清学技术鉴定为ABO亚型的25例标本,采用聚合酶链反应-序列特异性引物(PCR-SSP)和聚合酶链反应-测序分型(PCR-SBT)确定其基因型,对比分析其血清学和分子生物学检测结果。结果经血型血清学确认为ABO亚型的25例标本中,经分子生物学检测确证为亚型10例,其中B亚型7例,AB亚型3例。Bw11是主要ABO亚型等位基因(8/10)。另外血清学鉴定为亚型的15例经PCR-SBT法确认为非ABO亚型,其中以A102等位基因占比最高(9/15)。结论广西地区患者ABO亚型血清学与分子生物学结果一致率较低,宜选择合适的检测方法进行验证,以提高ABO亚型鉴定的准确性,保障临床输血安全。

Objective To investigate the serological and molecular biological characteristics of ABO subtypes in patients in Guangxi Zhuang Autonomous Region.Methods A total of 25 specimens in which ABO subtypes were identified by serologic tests were collected from the patients in Guangxi Zhuang Autonomous Region in 2021,and polymerase chain reaction-sequence specific primer(PCR-SSP)and polymerase chain reaction-sequence based typing(PCR-SBT)were used to determine the genotypes,and the detection results of serology and molecular biology were compared and analyzed.Results Of the 25 specimens confirmed to be ABO subtypes by serologic tests,10 cases were confirmed to be subtypes by molecular biological testing,including 7 cases of subtype B and 3 cases of subtype AB,and Bw11 was the major allele of ABO subtypes(8/10).The other 15 serological subtypes were confirmed as non-ABO subtypes by PCR-SBT method,among which the A102 allele was the most common(9/15).Conclusion The consistency rate between the serological and molecular biological results of ABO subtypes is low in patients in Guangxi Zhuang Autonomous Region.Appropriate detection methods should be selected for verification to improve the accuracy of ABO subtype identification and ensure the safety of clinical blood transfusion.