金荞麦收集系株型相关性状遗传变异分析

Genetic variation analysis of plant architecture-related traits of golden buckwheat accessions(Fagopyrum cymosum)

以金荞麦(Fagopyrum cymosum)为供试材料,对211份金荞麦收集系的4个株型相关性状(株高、主茎粗、主茎节数和主茎分枝数)进行了评价,并对其进行了相关性分析、主成分分析和聚类分析。结果显示:4个株型相关性状的变异系数为11.90%~19.05%。相关分析表明,株高与主茎粗(0.389**)、主茎节数(0.278**)、主茎分枝数(0.427**)呈极显著正相关,主茎粗与主茎节数(0.521**)、主茎分枝数(0.326**)呈极显著正相关,主茎节数与主茎分枝数(0.563**)呈极显著正相关。主成分分析将4个株型相关性状综合为3个主成分,前3个主成分累计贡献率达91.82%。系统聚类分析表明,211份金荞麦收集系可划分为7类,其中C1(包括29份供试材料)的株型相关性状较好,主要表现为:株高较高(152.85±5.04)cm、主茎较粗(6.13±0.70)mm、主茎节数最多(14.28±1.50)以及主茎分枝数较多(11.31±1.41),可作为优异的金荞麦种质资源进一步开发利用。

In this study, 211 golden buckwheat(Fagopyrum cymosum) accessions were used as experimental materials to evaluate four plant architecture-related traits, including plant height, stem diameter, number of main stem nodes and number of main stem branches, and the correlation analysis, principal component analysis(PCA)and cluster analysis were performed. The result showed that coefficient variation of the four tested traits ranged from 11.90% to 19.05%. The correlation analysis showed that the plant height was significantly positively correlated to the stem diameter(0.389**), number of main stem nodes(0.278**), and number of main stem branches(0.427**). The stem diameter was significantly positively correlated to the number of main stem nodes(0.521**)and the number of main stem branches(0.326**). The number of the main stem nodes was significantly positively correlated to the number of main stem branches(0.563**). PCA analysis divided the four traits into three principal components, which accounted for 91.82% of the total variation among the golden buckwheat accessions. The cluster analysis classified the 211 golden buckwheat accessions into seven distinct categories, among which, C1,including 29 accessions, had superior plant height(152.85 cm ± 5.04 cm), thicker stem diameter(6.13 mm ± 0.70mm), the most number of stem nodes(14.28 ± 1.50)and more main stem branches(11.31 ± 1.41) as compared with the other six categories. Therefore, accessions in C1 can be used as excellent golden buckwheat genetic resources for further exploitation and utilization.