聚乙烯亚胺/氧化石墨烯/苯丙氨酸手性界面对应激耐受多系分化细胞黏附和增殖的影响

Effects of chiral polyethyleneimine/graphene oxide/phenylalanine interface on adhesion and proliferation of multilineage-differentiating stress enduring cells

背景:近年来,应激耐受多系分化(Muse)细胞因其多能分化能力和非致瘤性而受到越来越多的关注,Muse细胞治疗在诸多疾病的动物实验中显示出较好的结果。然而,在长期的体外培养中,Muse细胞面临着增殖速度较慢的问题。目的:研究材料手性对Muse细胞黏附与增殖的影响。方法:通过层层自组装方法,以聚乙烯亚胺(PEI)作为基底层,氧化石墨烯(GO)和L/D-苯丙氨酸(L/D-Phe)顺序沉积形成聚乙烯亚胺/氧化石墨烯/苯丙氨酸手性生物界面(PEI/GO/L-Phe和PEI/GO/D-Phe)。研究自组装手性生物界面对Muse细胞黏附、增殖行为的影响。结果与结论:(1)通过简单的层层自组装技术构建了PEI/GO/L-Phe和PEI/GO/D-Phe手性界面;(2)统计结果显示:材料界面手性极大地影响了Muse细胞的行为,PEI/GO/L-Phe界面有利于Muse细胞的黏附和增殖,这可能由于该界面能吸附更多的蛋白,进一步促进了Muse细胞的增殖。

BACKGROUND:In recent years,multilineage-differentiating stress enduring(Muse) cell has attracted increasing attention due to its pluripotent differentiation capacity and non-tumorigenicity.Cell therapy based on Muse cells has shown exciting results in the animal experiments of many diseases.However,Muse cells face the difficulties of slow proliferation in long-term in vitro culture.OBJECTIVE:To investigate the effect of chirals interfaces on adhesion and proliferation of Muse cells.METHODS:The polyethyleneimine/graphene oxide/phenylalanine chiral biointerfaces(PEI/GO/L-Phe and PEI/GO/D-Phe) were constructed based on the layerby-layer self-assembly method using polyethyleneimine(PEI) as the substrate layer and graphene oxide(GO) and L/D-phenylalanine(L/D-Phe) sequentially deposited.The effect of self-assembled chiral biointerfaces on the adhesion and proliferation behavior of Muse cells was investigated.RESULTS AND CONCLUSION:(1) PEI/GO/L-Phe and PEI/GO/D-Phe chiral interfaces were successfully constructed based on facile self-assembly technology.(2)The statistical results showed that the chirality on the interface influenced Muse cell behaviors significantly.The initial adhesion and subsequent proliferation of Muse cells were enhanced by the PEI/GO/L-Phe interface,probably owing to the preferred adsorption of proteins,which further promote Muse cell proliferation.