巴戟天多糖对精索静脉曲张大鼠睾丸修复作用的蛋白质组学分析

Quantitative proteomic analyses of testis in varicocele rats treated with Morinda officinalis polysaccharide

目的:从蛋白质组学层面探讨精索静脉曲张(VC)的病理机制及巴戟天多糖(MOP)对VC大鼠睾丸修复作用的机制。方法:健康雄性SD大鼠随机分为假手术组、VC模型组、VC+MOP给药组。采用串联质谱标签法对大鼠左侧睾丸组织进行蛋白质组学分析,筛选差异表达蛋白(差异倍数>25%),并进行生物信息学分析。结果:VC模型组和假手术组相比差异蛋白总数为46个,VC+MOP给药组与VC模型组相比差异蛋白总数为21个,VC+MOP给药组与假手术相比差异蛋白总数为5个。差异表达蛋白层次聚类分析结果显示,VC+MOP给药组与假手术组之间蛋白表达相似,而VC模型组与其他2个组差异较大。基因本体(GO)和日本京都基因和基因组百科全书(KEGG)通路富集分析显示,差异蛋白主要富集于核糖体生物合成、细胞凋亡信号、激素水平调节、血液循环调节、体液免疫应答、蛋白激酶C信号转导、肽激素分泌通路。结论:MOP可使由VC引起的组间睾丸差异表达蛋白数量减少,逆转由VC引起的蛋白表达水平异常,提示VC的病理机制和MOP的修复作用可能与关键蛋白的差异表达以及富集的GO和KEGG通路有关。

Objective:To elucidate the pathophysiology of varicocele(VC)and the mechanism of repair effect of Morinda officinalis polysaccharide(MOP)on testis injury from the proteomic aspect.Methods:Healthy Sprague Dawley(SD)male rats were randomly divided into a sham group,a VC model group,and a VC+MOP treatment group.The Tandem Mass Tags-label based quantitative proteomic analyses of left testicular tissue were performed to evaluate the differentially expressed proteins(fold change>25%),followed by the bioinformatics analysis.Results:The number of differentially expressed proteins between the VC model group and sham group,the VC+MOP treatment group and VC model group,the VC+MOP treatment group and sham group was 46,21,and 5 respectively.The result of hierarchical clustering analysis of differentially expressed proteins showed that the protein expression of VC+MOP treatment group was similar to that of sham group,but the protein expression of VC model group was far different from that of the other two groups.The Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis showed that the differentially expressed proteins predominantly enrich on ribosome biogenesis,apoptotic signaling pathway,regulation of hormone levels,regulation of blood circulation,humoral immune response,regulation of protein kinase C signaling,regulation of peptide hormone secretion pathways.Conclusion:MOP can decrease the number and reverse the expression of differentially expressed proteins induced by VC in rat left testis.The differentially expressed proteins and their enriched GO and KEGG pathways may contribute to the VC pathophysiology and the MOP repair efficacy.