长非编码RNA在儿童肠息肉中的差异表达

Differential expression of long-stranded non-coding RNA in childhood intestinal polyps

目的探讨长非编码RNA(lncRNA)在儿童肠息肉的差异表达。方法以3例配对儿童正常肠黏膜和肠息肉,外加4例儿童肠息肉开展相关组织的lncRNA测序研究。随后分别在60例儿童肠息肉组织及正常肠黏膜组织标本运用RT-qPCR验证6个差异表达lncRNA基因。结果通过测序发现存在1307+738条差异表达的mRNA,共598条明显表达差异的lncRNA,其中上调472条,下调126条。RT-qPCR验证了MIR4435-2HG、SH3PXD2A-AS1、Lnc00668、MIR194-2HG、AC245060.6、BX890604.2等共6个基因。结论差异表达的lncRNA可能参与了儿童肠息肉的发病过程,这一现象拓展了对儿童肠息肉的认识,为临床诊治及预后判断提供了新的思路。

Objective To explore the differential expression of long-stranded non-coding RNA in child intestinal polyps.Methods The lncRNA sequencing study of the relevant tissues was carried out in 3 matched children with normal intestinal mucosa and intestinal polyps plus 4 children with intestinal polyps.Six differentially expressed lncRNA genes were subsequently validated respectively by RT-qPCR in 60 children’s intestinal polyp tissue and normal intestinal mucosa tissue specimens.Results The 1307+738 differentially expressed mRNAs were found by sequencing,and there were a total of 598 significantly differentially expressed lncRNAs,in which 472 were up-regulated and 126 were down-regulated.And RT-qPCR verified a total of 6 genes,including MIR4435-2 HG,SH3 PXD2 A-AS1,Lnc00668,MIR194-2 HG,AC245060.6,BX890604.2.Conclusion Differentially expressed lncRNAs may be involved in the pathogenesis of intestinal polyps in children,and this phenomenon may expand the understanding of intestinal polyps in children and provide new ideas for clinical diagnosis,treatment and prognosis judgment.