ADCY9基因与儿童支气管哮喘的易感性及环境交互作用分析

Association of the ADCY9 gene and gene-environmental interaction with the susceptibility to childhood bronchial asthma

目的 研究腺苷酸环化酶9 (adenylyl cyclase Ⅸ,ADCY9)基因rs1967309、rs2230739、rs2601814、rs2601825、rs2601796和rs2283497位点单核苷酸多态性(single nucleotide polymorphisms,SNPs)及基因-环境交互作用与儿童支气管哮喘(简称哮喘)的关系。方法 选取2019年3月至2021年9月就诊的哮喘儿童123例作为哮喘组,其中急性发作为轻中度84例(68.3%),重度39例(31.7%);124例健康体检儿童作为对照组。分析ADCY9基因6个位点SNPs及单倍型与儿童哮喘易感性的关系。同时采用广义多因子降维法分析基因-环境交互作用。结果 哮喘组和对照组儿童ADCY9基因6个位点存在多态性,其中rs1967309位点基因型及等位基因在哮喘组和对照组间的分布差异有统计学意义(P<0.05)。单倍型TA、GG在哮喘组和对照组的分布差异无统计学意义(P>0.05)。广义多因子降维法分析显示,rs1967309位点与变应原接触间存在交互作用(P<0.05),该位点与变应原接触的交互作用使哮喘患病风险增加(OR=1.585,P<0.05)。结论 ADCY9基因rs1967309位点与儿童哮喘易感性相关,且该位点和变应原接触具有协同致哮喘的作用。

Objective To study the association of the single nucleotide polymorphisms(SNPs) of the adenylyl cyclase Ⅸ(ADCY9) gene at rs1967309, rs2230739, rs2601814, rs2601825, rs2601796, and rs2283497 loci and gene-environment interaction with childhood bronchial asthma(asthma for short). Methods A total of 123 children with asthma who attended the hospital from March 2019 to September 2021 were enrolled as the asthma group, among whom 84(68.3%) had mild-to-moderate attacks and 39(31.7%) had severe attacks. A total of 124 healthy children were enrolled as the control group. The association of the SNPs and haplotypes of the ADCY9 gene at the above 6 loci with the susceptibility to childhood asthma was evaluated. The method of generalized multifactor dimensionality reduction was used to analyze gene-environment interaction. Results Polymorphisms were observed for the ADCY9 gene at the above six loci in both the asthma and control groups, and there were significant differences in genotype and allele frequencies at the rs1967309 locus between the two groups(P<0.05). There was no significant difference in the distribution frequency of haplotypes TA and GG between the asthma and control groups(P>0.05). The generalized multifactor dimensionality reduction analysis showed interaction between rs1967309 locus and allergen contact(P<0.05), which increased the risk of asthma(OR=1.585, P<0.05). Conclusions The rs1967309 locus of the ADCY9 gene is associated with the susceptibility to childhood asthma, and the locus and allergen contact have a synergistic effect on the development of asthma.