基于OPLS-DA结合熵权TOPSIS的不同产地益智仁综合质量评价

Comprehensive Quality Evaluation of Alpiniae Oxyphyllae Fructus from Different Producing Areas Based on OPLS-DA Combined with Entropy Weight TOPSIS Method

目的:对不同产地益智仁进行综合质量评价。方法:采用超高效液相色谱法建立15批不同产地益智仁的指纹图谱,确定共有峰,并进行相似度评价;以挥发油、水溶性浸出物、醇溶性浸出物、总灰分、酸不溶性灰分、二氧化硫残留量及指纹图谱为指标,采用正交偏最小二乘法-判别分析(OPLS-DA)和熵权逼近理想解排序法(TOPSIS)对益智仁的整体质量进行综合评价。结果:益智仁指纹图谱共标定了15个共有峰,指认了峰1、峰13分别为原儿茶酸、圆柚酮;OPLS-DA结果显示,峰4、峰11、峰13、峰14的峰面积及二氧化硫残留量是影响各产地益智仁质量差异的主要因素;熵权TOPSIS分析结果显示,海南省琼中县、屯昌县、万宁市,广东省阳春市、高州市益智仁质量评价贴近度(Ci)依次为0.520、0.378、0.577、0.206、0.207。结论:海南产益智仁质量整体优于广东产,且以海南省万宁市质量最优。OPLS-DA结合熵权TOPSIS构建的多指标综合评价模型,可排除主观因素的干扰,提高分析结果的科学性与准确性,可用于益智仁的整体质量评价。

Objective: To comprehensively evaluate the quality of Alpiniae Oxyphyllae Fructus from different producing areas. Methods: The ultra-high performance liquid chromatography was used to establish the fingerprint of15 batches of Alpiniae Oxyphyllae Fructus of different origins to determine the common peaks for the similarity evaluation. With volatile oil, alcoholsoluble extract, water-soluble extract, total ash, acid-insoluble ash, sulfur dioxide residue and fingerprint characteristic peaks as indices, orthogonal partial least squares discriminant analysis(OPLS-DA) and entropy weight technique for order preference by similarity to ideal solution(TOPSIS) method were used for comprehensive quality evaluation. Results: Fifteen common peaks were found, and peaks 1 and 13 were identified as protocatechuic acid and nootkatone, respectively. The OPLS-DA showed that the peak areas of peaks 4, nootkatone, 11 and 14 and sulfur dioxide residue were the main factors to distinguish Alpiniae Oxyphyllae Fructus of different origins. The entropy weight TOPSIS method demonstrated that the Civalues of Alpiniae Oxyphyllae Fructus in Hainan-Qiongzhong County, Hainan-Tunchang County, Hainan-Wanning City, Guangdong-Yangchun City and Guangdong-Gaozhou City were 0.520, 0.378, 0.577, 0.206 and 0.207, respectively.Conclusion: The quality of Alpiniae Oxyphyllae Fructus from Hainan was better than that from Guangdong, and Wanning City was the best producing area. The multiindex comprehensive evaluation model constructed by OPLS-DA combined with entropy weight TOPSIS method can eliminate the interference of subjective factors and improve the scientificity and accuracy of the analysis results, and can be used for the overall quality evaluation of Alpiniae Oxyphyllae Fructus.