Cariporide对癫痫模型大鼠心肌细胞凋亡的影响

Effect of cariporide on cardiomyocyte apoptosis in epileptic rats

目的 探讨Cariporide对癫痫模型大鼠心肌细胞凋亡的影响。方法 选取SD雄性大鼠45只,氯化锂—匹罗卡品构建癫痫模型作为模型组(n=30),腹腔注射生理盐水作为对照组(n=15),采用Cariporide干预癫痫模型鼠作为干预组(n=15)。第60天麻醉处死大鼠后取心肌组织,应用蛋白印迹和RT-PCR检测钠氢交换体1(NHE-1)的表达,采用流式细胞检测大鼠心肌细胞凋亡情况。结果 制作模型过程中7只大鼠死亡,死亡率为23%。第60天蛋白印迹结果显示干预组大鼠较模型组心肌细胞NHE-1蛋白表达降低(P<0.05)。RT-PCR结果显示干预组大鼠较模型组心肌细胞NHE-1 mRNA表达降低(P<0.05)。流式细胞检测显示干预组大鼠较模型组心肌细胞凋亡率明显降低(P<0.05)。结论 Cariporide可通过下调NHE-1来改善癫痫模型大鼠心肌细胞凋亡情况。

Objective To investigate the effect of Cariporide on cardiomyocyte apoptosis in rat epilepsy model. Methods Forty-five male SD rats were selected. The rats were injected with lithium chloride pilocarpine as model group(n=30), and the rats were intraperitoneally injected normal saline as control group(n=15). Cariporide was used to intervene rats with epilepsy as an intervention group(n=15). On the 60day, the rats were anesthetized and sacrificed, and myocardial tissues were collected. Western blot and RT-PCR were used to detect the expression of sodium-hydrogen exchanger isoform-1(NHE-1). Flow cytometry was used to detect the apoptosis of rat cardiomyocytes. Results Seven rats died in the process of establishing the model, and the mortality rate was 23%. On the 60 th day, Western blot showed that NHE-1 protein expression in cardiomyocyte of intervention group was lower than that of the model group(P<0.05). RT-PCR results showed that NHE-1 mRNA expression in the cardiomyocyte was lower in intervention group than that in model group(P<0.05). Flow cytometry showed that the apoptosis rate of cardiomyocyte in intervention group was significantly lower than that in model group(P<0.05). Conclusion Cariporide can improve cardiomyocyte apoptosis by downregulating NHE-1 in rat epilepsy model.