大肠埃希氏菌和蜡样芽孢杆菌致犊牛腹泻的诊治

Diagnosis and Treatment of Calf Diarrhea Caused by E.coli and Bacillus cereus

为了对某肉牛场发生的犊牛腹泻进行诊断和治疗,采集了发病犊牛的肝脏和脾脏,通过细菌分离鉴定和小鼠致病性试验对其进行诊断。最终分离到1株蜡样芽孢杆菌和1株致病性大肠埃希氏菌,致病性试验发现所分离的蜡样芽孢杆菌和致病性大肠埃希氏菌接种小鼠会使其分别在36 h和24 h全部死亡。同时,结合发病犊牛的临床表现确诊为致病性大肠埃希氏菌混合蜡样芽孢杆菌感染引起的腹泻。药敏试验结果显示致病性大肠埃希氏菌对头孢噻呋钠、硫酸庆大霉素和复方磺胺氯达嗪钠敏感,蜡样芽孢杆菌对庆大霉素、氟苯尼考、万古霉素和恩诺沙星敏感。对发病犊牛进行隔离,参考药敏试验结果配合中药处方等措施,犊牛病情得到有效控制。

To diagnose and treat the claf diarrhea occurred in a beef cattle farm, the livers and spleens were collected from diarrhea calves for pathogenic bacteria isolation.The molecular biology diagnosis and animal pathogenicity test were performed to identify the isolates.Finally, we got 1 B.cereus isolate and 1 pathogenic E.coli isolate.Pathogenicity test revealed the B.cereus and the pathogenic E.coli isolates could cause mice death at 36 h and 24 h, respectively.Combined with clinical symptoms, the results revealed that pathogenic E.coli and B.cereus were responsible for the claf diarrhea.The antimicrobial susceptibility test revealed the pathogenic E.coli and Bacillus were sensitive to ceftiofur sodium, gentamicin sulfate, compound sulfachlordazine sodium, gentamicin, florfenicol, vancomycin and enrofloxacin, respectively.The disease was effectively controlled by isolating diseased calves and treating with the sensitive drugs and herbal prescription.